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Chemiluminescence Immunoassays for Simultaneous Detection of Three Heart Disease Biomarkers Using Magnetic Carbon Composites and Three-Dimensional Microfluidic Paper-Based Device
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-09-30 00:00:00 , DOI: 10.1021/acs.analchem.9b03066 Rui Yang 1 , Fang Li 2 , Wencan Zhang 1 , Wen Shen 1 , Di Yang 3 , Zhiping Bian 3 , Hua Cui 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-09-30 00:00:00 , DOI: 10.1021/acs.analchem.9b03066 Rui Yang 1 , Fang Li 2 , Wencan Zhang 1 , Wen Shen 1 , Di Yang 3 , Zhiping Bian 3 , Hua Cui 1
Affiliation
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Chemiluminescence (CL) immunoassays for simultaneous detection of early acute myocardial infarction (AMI) biomarkers, including copeptin, heart-type fatty acid binding protein (h-FABP), and cardiac troponin I (cTnI), were developed by using Co2+/N-(aminobutyl)-N-(ethylisoluminol) (ABEI) functionalized magnetic carbon composite (Co2+-ABEI-Fe3O4@void@C) as an interface and a three-dimensional microfluidic paper-based device (3D μPAD) as a detection system. For CL immunoassays, Co2+-ABEI-Fe3O4@void@C was assembled with chitosan (CS) and gold nanoparticle-conjugated antibody (Au-Ab) sequentially to form the sensing platform (Co2+-ABEI-Fe3O4@void@C-CS/Au-Ab). In the presence of antigen (Ag), Ag was captured by the sensing interface to form an immunocomplex, leading to an increase in CL intensity due to the catalysis of −COO– existing in Ag. A 3D μPAD with three detection zones for simultaneous detection of copeptin, h-FABP, and cTnI was designed and fabricated to obtain time-resolved CL signals. Three kinds of immunocomplexes formed with copeptin, h-FABP, and cTnI were added to three detection zones of 3D μPAD, respectively. After injecting H2O2, three time-resolved CL signals were generated in one CL detection run by virtue of time-delayed transport of H2O2 to different detection zones. The three time-resolved CL signals were used for the simultaneous determination of copeptin, h-FABP, and cTnI. The detection limit of copeptin, h-FABP, and cTnI was 0.40 pg/mL, 0.32 pg/mL, and 0.50 pg/mL, respectively, which is at least 1 order of magnitude lower than most of the reported immunoassays. The immunoassays could be directly used for the detection of copeptin, h-FABP, and cTnI in human serum samples. The proposed immunoassays are simple, fast, sensitive, and selective, and are of great application potential in early diagnosis and treatment of AMI.
中文翻译：
化学发光免疫分析同时使用磁性碳复合物和三维微流控纸基设备检测三种心脏病生物标志物
通过使用Co 2+ /开发了用于同时检测早期急性心肌梗死（AMI）生物标志物的化学发光（CL）免疫测定法，包括生物素肽，心脏型脂肪酸结合蛋白（h-FABP）和心肌肌钙蛋白I（cTnI）。N-（氨基丁基）-N-（乙基异鲁米诺）（ABEI）功能化的磁性碳复合材料（Co 2+ -ABEI-Fe 3 O 4 @ void @ C）作为界面和三维微流体纸基设备（3DμPAD ）作为检测系统。对于CL免疫测定，Co 2+ -ABEI-Fe 3 O 4@ void @ C与壳聚糖（CS）和金纳米颗粒偶联抗体（Au-Ab）依次组装以形成传感平台（Co 2+ -ABEI-Fe 3 O 4 @ void @ C-CS / Au-Ab） 。在抗原（Ag）的存在，银是由感应接口捕获以形成免疫复合物，导致增加CL强度由于-COO的催化-存在于银。设计并制造了具有三个检测区的3DμPAD，用于同时检测肽素，h-FABP和cTnI，以获得时间分辨的CL信号。将由肽素，h-FABP和cTnI形成的三种免疫复合物分别添加到3DμPAD的三个检测区域中。注入H 2 O 2后，通过将H 2 O 2延迟传输到不同的检测区域，在一次CL检测运行中生成了三个时间分辨的CL信号。三个时间分辨的CL信号用于同时测定肽素，h-FABP和cTnI。copeptin，h-FABP和cTnI的检出限分别为0.40 pg / mL，0.32 pg / mL和0.50 pg / mL，比大多数报告的免疫测定低至少1个数量级。免疫测定可直接用于检测人血清样品中的肽素，h-FABP和cTnI。所提出的免疫测定方法简单，快速，灵敏和选择性强，在AMI的早期诊断和治疗中具有巨大的应用潜力。
更新日期：2019-09-30
中文翻译：
化学发光免疫分析同时使用磁性碳复合物和三维微流控纸基设备检测三种心脏病生物标志物
通过使用Co 2+ /开发了用于同时检测早期急性心肌梗死（AMI）生物标志物的化学发光（CL）免疫测定法，包括生物素肽，心脏型脂肪酸结合蛋白（h-FABP）和心肌肌钙蛋白I（cTnI）。N-（氨基丁基）-N-（乙基异鲁米诺）（ABEI）功能化的磁性碳复合材料（Co 2+ -ABEI-Fe 3 O 4 @ void @ C）作为界面和三维微流体纸基设备（3DμPAD ）作为检测系统。对于CL免疫测定，Co 2+ -ABEI-Fe 3 O 4@ void @ C与壳聚糖（CS）和金纳米颗粒偶联抗体（Au-Ab）依次组装以形成传感平台（Co 2+ -ABEI-Fe 3 O 4 @ void @ C-CS / Au-Ab） 。在抗原（Ag）的存在，银是由感应接口捕获以形成免疫复合物，导致增加CL强度由于-COO的催化-存在于银。设计并制造了具有三个检测区的3DμPAD，用于同时检测肽素，h-FABP和cTnI，以获得时间分辨的CL信号。将由肽素，h-FABP和cTnI形成的三种免疫复合物分别添加到3DμPAD的三个检测区域中。注入H 2 O 2后，通过将H 2 O 2延迟传输到不同的检测区域，在一次CL检测运行中生成了三个时间分辨的CL信号。三个时间分辨的CL信号用于同时测定肽素，h-FABP和cTnI。copeptin，h-FABP和cTnI的检出限分别为0.40 pg / mL，0.32 pg / mL和0.50 pg / mL，比大多数报告的免疫测定低至少1个数量级。免疫测定可直接用于检测人血清样品中的肽素，h-FABP和cTnI。所提出的免疫测定方法简单，快速，灵敏和选择性强，在AMI的早期诊断和治疗中具有巨大的应用潜力。
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