循环 microRNA 谱作为阻塞性睡眠呼吸暂停诊断的潜在生物标志物。,Scientific Reports

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循环 microRNA 谱作为阻塞性睡眠呼吸暂停诊断的潜在生物标志物。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-09-17 , DOI: 10.1038/s41598-019-49940-1
Fernando Santamaria-Martos ₁ , Iván Benítez _{1,

2} , Francisco Ortega _{3,

4} , Andrea Zapater ₁ , Cristina Giron ₁ , Lucía Pinilla ₁ , Lydia Pascual ₁ , Anunciación Cortijo ₁ , Mireia Dalmases ₁ , Jose M Fernandez-Real _{3,

4} , Ferran Barbé _{1,

2} , Manuel Sánchez-de-la-Torre _{1,

2}

Affiliation

对 microRNA (miRNA) 的评估可以表征阻塞性睡眠呼吸暂停 (OSA) 并帮助更准确地诊断。我们的目的是检查循环 miRNA 谱，以确定非 OSA 患者和 OSA 患者之间的差异。此外，我们的目的是分析持续气道正压通气 (CPAP) 治疗对 miRNA 谱的影响。这项观察性纵向研究纳入了 230 名因疑似 OSA 转入睡眠科的受试者。通过 TaqMan-Low-Density-Array 对 27 名受试者的血浆中 188 种 miRNA 进行了表达谱分析。选择 OSA 相关 miRNA 在 203 名患者中通过 RT-qPCR 进行验证。建立预测模型来区分非 OSA 和 OSA：1) NoSAS 评分，2) 差异表达的 miRNA，以及 3) NoSAS 评分加 miRNA 的组合。随访6个月后测量差异表达的miRNA。从选择用于验证的 14 个 miRNA 中，有 6 个被确认为差异表达。NoSAS 评分的曲线下面积为 0.73，miRNA 的曲线下面积为 0.81，组合的曲线下面积为 0.86。经过 6 个月的 CPAP 治疗后，OSA 组的 miRNA 水平似乎接近非 OSA 水平。一组 miRNA 被鉴定用于区分非 OSA 和 OSA 患者。CPAP 治疗与循环 miRNA 谱的变化相关。

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Circulating microRNA profile as a potential biomarker for obstructive sleep apnea diagnosis.

Evaluation of microRNAs (miRNAs) could allow characterization of the obstructive sleep apnea (OSA) and help diagnose it more accurately. We aimed to examine circulating miRNA profiles to establish the differences between non-OSA and OSA patients. Additionally, we aimed to analyse the effect of continuous positive airway pressure (CPAP) treatment on the miRNA profile. This observational, longitudinal study included 230 subjects referred to the Sleep Unit due to suspected OSA. Expression profiling of 188 miRNAs in plasma was performed in 27 subjects by TaqMan-Low-Density-Array. OSA-related miRNAs were selected for validation by RT-qPCR in 203 patients. Prediction models were built to discriminate between non-OSA and OSA: 1) NoSAS-score, 2) differentially expressed miRNAs, and 3) combination of NoSAS-score plus miRNAs. The differentially expressed miRNAs were measured after 6 months of follow-up. From the 14 miRNAs selected for validation, 6 were confirmed to be differentially expressed. The areas under the curve were 0.73 for the NoSAS-score, 0.81 for the miRNAs and 0.86 for the combination. After 6 months of CPAP treatment, miRNA levels in the OSA group seem to approximate to non-OSA levels. A cluster of miRNAs was identified to differentiate between non-OSA and OSA patients. CPAP treatment was associated with changes in the circulating miRNA profile.

更新日期：2019-09-18

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