催化发夹组装反应在生物传感中的应用。,Small

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催化发夹组装反应在生物传感中的应用。
Small ( IF 13.0 ) Pub Date : 2019-09-16 , DOI: 10.1002/smll.201902989
Jumei Liu ₁ , Ye Zhang ₂ , Huabin Xie ₃ , Li Zhao ₄ , Lei Zheng ₂ , Huiming Ye _{1,

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Affiliation

核酸被认为是用于级联信号放大的完美可编程材料，而不仅仅是遗传信息载体。其中，典型的例子是无酶，高效，等温扩增的催化发夹装配体（CHA）。典型的CHA反应由单链分析物引发，底物发夹依次打开，从而形成热力学稳定的双链体。CHA电路于2008年首次提出，如今已发展成数十个系统。通过对机理的深入研究，CHA电路不断丰富了各种反应系统并提高了分析性能。短时间后，CHA反应可在等温条件下实现指数扩增。在某些情况下，CHA反应甚至可以实现60万倍的信号放大。由于其前景广阔的多功能性，CHA可以用于体外和活细胞中各种标记的分析。此外，CHA还与纳米材料和其他分子生物技术集成在一起，以产生各种读数。在此，详细介绍了各种CHA机制，发夹设计和反应条件。此外，提出了基于CHA的生物传感器。最后，考虑了CHA的挑战和发展前景。详细介绍了反应条件。此外，提出了基于CHA的生物传感器。最后，考虑了CHA的挑战和发展前景。详细介绍了反应条件。此外，提出了基于CHA的生物传感器。最后，考虑了CHA的挑战和发展前景。

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Applications of Catalytic Hairpin Assembly Reaction in Biosensing.

Nucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme-free, high-efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single-stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in-depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000-fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.

更新日期：2019-10-16

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