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NIR-II-Excited Intravital Two-Photon Microscopy Distinguishes Deep Cerebral and Tumor Vasculatures with an Ultrabright NIR-I AIE Luminogen.
Advanced Materials ( IF 27.4 ) Pub Date : 2019-09-15 , DOI: 10.1002/adma.201904447 Shaowei Wang 1 , Jie Liu 2 , Chi Ching Goh 3 , Lai Guan Ng 3 , Bin Liu 1
Advanced Materials ( IF 27.4 ) Pub Date : 2019-09-15 , DOI: 10.1002/adma.201904447 Shaowei Wang 1 , Jie Liu 2 , Chi Ching Goh 3 , Lai Guan Ng 3 , Bin Liu 1
Affiliation
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Intravital fluorescence imaging of vasculature morphology and dynamics in the brain and in tumors with large penetration depth and high signal-to-background ratio (SBR) is highly desirable for the study and theranostics of vascular-related diseases and cancers. Herein, a highly bright fluorophore (BTPETQ) with long-wavelength absorption and aggregation-induced near-infrared (NIR) emission (maximum at ≈700 nm) is designed for intravital two-photon fluorescence (2PF) imaging of a mouse brain and tumor vasculatures under NIR-II light (1200 nm) excitation. BTPETQ dots fabricated via nanoprecipitation show uniform size of around 42 nm and a high quantum yield of 19 ± 1% in aqueous media. The 2PF imaging of the mouse brain vasculatures labeled by BTPETQ dots reveals a 3D blood vessel network with an ultradeep depth of 924 µm. In addition, BTPETQ dots show enhanced 2PF in tumor vasculatures due to their unique leaky structures, which facilitates the differentiation of normal blood vessels from tumor vessels with high SBR in deep tumor tissues. Moreover, the extravasation and accumulation of BTPETQ dots in deep tumor (more than 900 µm) is visualized under NIR-II excitation. This study highlights the importance of developing NIR-II light excitable efficient NIR fluorophores for in vivo deep tissue and high contrast tumor imaging.
中文翻译：
NIR-II激发的玻璃体内双光子显微镜用超亮NIR-I AIE发光剂区分深部脑和肿瘤血管。
对于血管相关疾病和癌症的研究和治疗学，非常需要在颅内以及大穿透深度和高信噪比（SBR）的肿瘤中进行脉管内形态和动力学的玻璃体内荧光成像。本文中，设计了一种具有长波长吸收和聚集诱导的近红外（NIR）发射（最大≈700nm）的高亮度荧光团（BTPETQ），用于小鼠脑和肿瘤的活体双光子荧光（2PF）成像NIR-II光（1200 nm）激发下的脉管系统。通过纳米沉淀法制备的BTPETQ点在水性介质中显示出约42 nm的均匀大小和19±1％的高量子产率。用BTPETQ点标记的小鼠脑血管的2PF成像显示了3D血管网络，其超深深度为924 µm。此外，BTPETQ点由于其独特的泄漏结构而在肿瘤血管中显示出增强的2PF，这有助于正常血管与深部肿瘤组织中具有高SBR的肿瘤血管的区分。此外，在NIR-II激发下，可以看到BTPETQ点在深部肿瘤（大于900 µm）中的扩散和积累。这项研究强调了开发NIR-II光可激发的有效NIR荧光团对于体内深层组织和高对比度肿瘤成像的重要性。
更新日期：2019-11-01
中文翻译：
NIR-II激发的玻璃体内双光子显微镜用超亮NIR-I AIE发光剂区分深部脑和肿瘤血管。
对于血管相关疾病和癌症的研究和治疗学，非常需要在颅内以及大穿透深度和高信噪比（SBR）的肿瘤中进行脉管内形态和动力学的玻璃体内荧光成像。本文中，设计了一种具有长波长吸收和聚集诱导的近红外（NIR）发射（最大≈700nm）的高亮度荧光团（BTPETQ），用于小鼠脑和肿瘤的活体双光子荧光（2PF）成像NIR-II光（1200 nm）激发下的脉管系统。通过纳米沉淀法制备的BTPETQ点在水性介质中显示出约42 nm的均匀大小和19±1％的高量子产率。用BTPETQ点标记的小鼠脑血管的2PF成像显示了3D血管网络，其超深深度为924 µm。此外，BTPETQ点由于其独特的泄漏结构而在肿瘤血管中显示出增强的2PF，这有助于正常血管与深部肿瘤组织中具有高SBR的肿瘤血管的区分。此外，在NIR-II激发下，可以看到BTPETQ点在深部肿瘤（大于900 µm）中的扩散和积累。这项研究强调了开发NIR-II光可激发的有效NIR荧光团对于体内深层组织和高对比度肿瘤成像的重要性。
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