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Signal Production and Response Specificity in the phc Quorum Sensing Systems of Ralstonia solanacearum Species Complex.
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2019-09-24 , DOI: 10.1021/acschembio.9b00553 Yumeto Ujita 1 , Megumi Sakata 1 , Ayaka Yoshihara 1 , Yasufumi Hikichi 2 , Kenji Kai 1
ACS Chemical Biology ( IF 3.5 ) Pub Date : 2019-09-24 , DOI: 10.1021/acschembio.9b00553 Yumeto Ujita 1 , Megumi Sakata 1 , Ayaka Yoshihara 1 , Yasufumi Hikichi 2 , Kenji Kai 1
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Ralstonia solanacearum strains are devastating plant pathogens with global distribution, a wide host range, and genetic diversity, and they are now also referred to as the R. solanacearum species complex (RSSC). RSSC strains employ the quorum sensing (QS) system composed of the phcBSR operon to regulate their virulence on plants. The RSSC strains previously examined produce either (R)-methyl 3-hydroxymyristate (3-OH MAME) or (R)-methyl 3-hydroxypalmitate (3-OH PAME) as their QS signals. Analogously, the phylogenetic analyses of the signal synthase PhcB and the signal receptor PhcS from 15 RSSC strains revealed that these proteins have two clades dependent on their QS signal types. However, the biochemical mechanism underlying this selectivity of QS signal production remains to be elucidated. We demonstrated that the PhcB methyltransferases synthesize QS signals from the cognate fatty acids (R)-3-hydroxymyristic acid or (R)-3-hydroxypalmitic acid. The RSSC strains used here produced both fatty acids, and thus the selectivity of QS signal production depends on the activity of PhcB enzymes. On the other hand, the enantioselective supply of the precursors functioned in the production of enantiopure QS signals. The opposite QS signals weakly induced the production of virulence factors in the RSSC strains. Furthermore, the complementation of the phcB gene encoding the 3-OH PAME-type synthase to the phcB-deletion mutant of the 3-OH MAME-producing strain did not rescue its virulence on tomato plants. Taken together, we propose that the specific production of 3-OH MAME/3-OH PAME ensures full virulence of the RSSC strains.
中文翻译：
Ralstonia solanacearum物种复合体的phc群体感应系统中的信号产生和响应特异性。
Ralstonia solanacearum菌株是具有全球分布，广泛寄主范围和遗传多样性的毁灭性植物病原体，现在也被称为solanacearum物种复合体（RSSC）。RSSC菌株采用由phcBSR操纵子组成的群体感应（QS）系统来调节其对植物的毒力。先前检查过的RSSC菌株产生（R）-3-羟基肉豆蔻酸甲酯（3-OH MAME）或（R）-3-羟基棕榈酸甲酯（3-OH PAME）作为其QS信号。类似地，对来自15个RSSC菌株的信号合酶PhcB和信号受体PhcS的系统发育分析表明，这些蛋白质根据其QS信号类型具有两个进化枝。然而，仍需要阐明这种QS信号产生选择性的生化机制。我们证明了PhcB甲基转移酶从同源脂肪酸（R）-3-羟基肉豆蔻酸或（R）-3-羟基棕榈酸合成QS信号。这里使用的RSSC菌株产生两种脂肪酸，因此QS信号产生的选择性取决于PhcB酶的活性。另一方面，前体的对映选择性供应在产生对映纯QS信号中起作用。相反的QS信号弱诱导RSSC菌株中毒力因子的产生。此外，编码3-OH PAME型合酶的phcB基因与产生3-OH MAME的菌株的phcB-缺失突变体的互补不能挽救其对番茄植物的毒力。两者合计，我们建议3-OH MAME / 3-OH PAME的特定生产确保RSSC菌株的完全毒力。
更新日期：2019-09-25
中文翻译：
Ralstonia solanacearum物种复合体的phc群体感应系统中的信号产生和响应特异性。
Ralstonia solanacearum菌株是具有全球分布，广泛寄主范围和遗传多样性的毁灭性植物病原体，现在也被称为solanacearum物种复合体（RSSC）。RSSC菌株采用由phcBSR操纵子组成的群体感应（QS）系统来调节其对植物的毒力。先前检查过的RSSC菌株产生（R）-3-羟基肉豆蔻酸甲酯（3-OH MAME）或（R）-3-羟基棕榈酸甲酯（3-OH PAME）作为其QS信号。类似地，对来自15个RSSC菌株的信号合酶PhcB和信号受体PhcS的系统发育分析表明，这些蛋白质根据其QS信号类型具有两个进化枝。然而，仍需要阐明这种QS信号产生选择性的生化机制。我们证明了PhcB甲基转移酶从同源脂肪酸（R）-3-羟基肉豆蔻酸或（R）-3-羟基棕榈酸合成QS信号。这里使用的RSSC菌株产生两种脂肪酸，因此QS信号产生的选择性取决于PhcB酶的活性。另一方面，前体的对映选择性供应在产生对映纯QS信号中起作用。相反的QS信号弱诱导RSSC菌株中毒力因子的产生。此外，编码3-OH PAME型合酶的phcB基因与产生3-OH MAME的菌株的phcB-缺失突变体的互补不能挽救其对番茄植物的毒力。两者合计，我们建议3-OH MAME / 3-OH PAME的特定生产确保RSSC菌株的完全毒力。
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