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Bacterial Extracellular Electron Transfer Occurs in Mammalian Gut
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-09-18 , DOI: 10.1021/acs.analchem.9b03176 Wei Wang 1 , Yahui Du 2 , Shuai Yang 1 , Xiaochen Du 1 , Min Li 1 , Bingqian Lin 2 , Jie Zhou 3 , Liyuan Lin 1 , Yanling Song 1 , Juan Li 1, 3 , Xiaolei Zuo 1 , Chaoyong Yang 1, 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-09-18 , DOI: 10.1021/acs.analchem.9b03176 Wei Wang 1 , Yahui Du 2 , Shuai Yang 1 , Xiaochen Du 1 , Min Li 1 , Bingqian Lin 2 , Jie Zhou 3 , Liyuan Lin 1 , Yanling Song 1 , Juan Li 1, 3 , Xiaolei Zuo 1 , Chaoyong Yang 1, 2
Affiliation
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As a well-studied biochemical reduction process in environmental microbiology, extracellular electron transfer (EET) was recently discovered in bacteria closely related to human health, and orthologues of a flavin-based EET gene were found in the genomes of many species across Firmicutes, a major phylum in mammalian gut microbiota. However, EET has not yet been confirmed to occur in mammalian gut, the presence of which may have broad physiological influences. Toward this end, here we first confirmed the occurrence of EET in mouse gut microbiotas cultured in vitro. Cyclic voltammetry analysis was then performed by directly inserting electrodes into the mouse cecum under anaerobic conditions, and a characteristic catalytic wave was observed in the gut of conventional but not germ-free mouse, proving the existence of in vivo bacterial EET. We also detected similar catalytic waves in the cecal microbiotas of rat and guinea pig in vivo, suggesting EET’s high prevalence in mammalian intestines. Our finding on the bacterial electron production in mammalian guts offers a new bioelectrochemical scope for deciphering the complex microbiology of gut bacteria and its effects on host physiology.
中文翻译：
哺乳动物肠内发生细菌细胞外电子转移
作为环境微生物学中经过深入研究的生化还原过程，最近在与人类健康密切相关的细菌中发现了细胞外电子转移（EET），并且在Firmicutes的许多物种的基因组中发现了基于黄素的EET基因的直向同源物。哺乳动物肠道菌群中的主要门。然而，尚未证实EET在哺乳动物肠道中发生，其存在可能具有广泛的生理影响。为此，我们首先在体外培养的小鼠肠道菌群中证实了EET的发生。。然后通过在厌氧条件下将电极直接插入小鼠盲肠中进行循环伏安分析，并在常规而非无菌小鼠的肠道中观察到特征性催化波，证明了体内细菌EET的存在。我们还在体内的大鼠和豚鼠的盲肠微生物群中检测到类似的催化波，这表明EET在哺乳动物肠道中的患病率很高。我们对哺乳动物肠道中细菌电子产生的发现为破译肠道细菌的复杂微生物及其对宿主生理的影响提供了新的生物电化学范围。
更新日期：2019-09-18
中文翻译：
哺乳动物肠内发生细菌细胞外电子转移
作为环境微生物学中经过深入研究的生化还原过程，最近在与人类健康密切相关的细菌中发现了细胞外电子转移（EET），并且在Firmicutes的许多物种的基因组中发现了基于黄素的EET基因的直向同源物。哺乳动物肠道菌群中的主要门。然而，尚未证实EET在哺乳动物肠道中发生，其存在可能具有广泛的生理影响。为此，我们首先在体外培养的小鼠肠道菌群中证实了EET的发生。。然后通过在厌氧条件下将电极直接插入小鼠盲肠中进行循环伏安分析，并在常规而非无菌小鼠的肠道中观察到特征性催化波，证明了体内细菌EET的存在。我们还在体内的大鼠和豚鼠的盲肠微生物群中检测到类似的催化波，这表明EET在哺乳动物肠道中的患病率很高。我们对哺乳动物肠道中细菌电子产生的发现为破译肠道细菌的复杂微生物及其对宿主生理的影响提供了新的生物电化学范围。
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