Nanoparticle Size Influences Antigen Retention and Presentation in Lymph Node Follicles for Humoral Immunity.,Nano Letters

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Nanoparticle Size Influences Antigen Retention and Presentation in Lymph Node Follicles for Humoral Immunity.
Nano Letters ( IF 9.6 ) Pub Date : 2019-09-17 , DOI: 10.1021/acs.nanolett.9b02834
Yi-Nan Zhang _{1,

2} , James Lazarovits _{1,

2} , Wilson Poon _{1,

2} , Ben Ouyang _{1,

2,

3} , Luan N M Nguyen _{1,

2} , Benjamin R Kingston _{1,

2} , Warren C W Chan _{1,

2,

4,

5,

6}

Affiliation

Lymph node follicles capture and retain antigens to induce germinal centers and long-lived humoral immunity. However, control over antigen retention has been limited. Here we discovered that antigen conjugated to nanoparticle carriers of different sizes impacts the intralymph node transport and specific cell interaction. We found that follicular dendritic cell (FDC) networks determine the intralymph node follicle fate of these nanoparticles by clearing smaller ones (5-15 nm) within 48 h and retaining larger ones (50-100 nm) for over 5 weeks. The 50-100 nm-sized nanoparticles had 175-fold more delivery of antigen at the FDC dendrites, 5-fold enhanced humoral immune responses of germinal center B cell formation, and 5-fold more antigen-specific antibody production over 5-15 nm nanoparticles. Our results show that we can tune humoral immunity by simply manipulating the carrier size design to produce effectiveness of vaccines.

中文翻译：

纳米颗粒的大小影响抗原保留和淋巴结滤泡的体液免疫表现。

淋巴结滤泡捕获并保留抗原以诱导生发中心和长寿命的体液免疫。但是，对抗原保留的控制受到限制。在这里，我们发现与不同大小的纳米颗粒载体缀合的抗原会影响淋巴结内转运和特异性细胞相互作用。我们发现，滤泡树突状细胞（FDC）网络通过在48小时内清除较小的颗粒（5-15 nm）并保留较大的颗粒（50-100 nm）超过5周来确定这些纳米颗粒的淋巴结内卵泡命运。50-100 nm大小的纳米颗粒在FDC树突上的抗原递送量增加了175倍，生发中心B细胞形成的体液免疫应答提高了5倍，在5-15 nm范围内产生的抗原特异性抗体增加了5倍纳米粒子。

更新日期：2019-09-18

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