当前位置：
X-MOL 学术
›
ACS Appl. Bio Mater.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
In Situ Forming and Reversibly Cross-Linkable Hydrogels Based on Copolypept(o)ides and Polysaccharides
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2019-09-25 , DOI: 10.1021/acsabm.9b00668 Yanping Tong 1 , Zhaochuang Wang 1 , Yan Xiao 1 , Wei Liu 2 , Jinghao Pan 1 , Yan Zhou 2 , Meidong Lang 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2019-09-25 , DOI: 10.1021/acsabm.9b00668 Yanping Tong 1 , Zhaochuang Wang 1 , Yan Xiao 1 , Wei Liu 2 , Jinghao Pan 1 , Yan Zhou 2 , Meidong Lang 1
Affiliation
|
The emerging tide of hydrogels in biomedical fields drives them to possess good biocompatibility, tunable mechanical properties, and fast gelation process. Herein, a composite hydrogel containing copolypept(o)ides and functional polysaccharides was constructed through dynamic acylhydrazone linkages. First, a series of peptide–peptoid copolymers were synthesized by ring-opening polymerization of sarcosine (Sar) and l-glutamic acid γ-benzyl ester (BLG) N-carboxyanhydrides (NCAs). The benzyl groups of BLG units were substituted with hydrazide groups through ester–amide exchange aminolysis reaction. The statistical copolymer of poly(sarcosine-co-glutamate-hydrazide) (P(Sar-co-GH)) was chosen as an optimized precursor due to its excellent water solubility and gel-forming ability with aldehyde-modified sodium alginate (OSA). Moreover, cellulose nanocrystals (CNCs) were prepared as nanofillers to reinforce the P(Sar-co-GH)-OSA hydrogel. We demonstrated that the copolymer sequences and composition contents made a difference to the properties of the formed hydrogels by variation of the cross-linking density. The dynamic acylhydrazone bonds endowed hydrogels with pH responsiveness and reversible networks. The NIH/3T3 cells encapsulated in the hydrogels maintained high viability and proliferation abilities, indicating that the nanocomposite hydrogels could be explored to fabricate a customized responsive drug delivery system or cell scaffolds for tissue engineering.
中文翻译：
基于共聚肽(o)ides和多糖的原位形成和可逆交联水凝胶
生物医学领域水凝胶的兴起促使它们具有良好的生物相容性、可调节的机械性能和快速的凝胶过程。在此，通过动态酰基腙键构建了一种含有共聚肽(o)ides 和功能性多糖的复合水凝胶。首先，通过肌氨酸 (Sar) 和l-谷氨酸 γ-苄酯 (BLG) N-羧酸酐 (NCA)的开环聚合合成了一系列肽-类肽共聚物。BLG单元的苄基通过酯-酰胺交换氨解反应被酰肼基团取代。聚（肌氨酸-co-谷氨酸-酰肼）的统计共聚物（P（Sar- co-GH)) 因其优异的水溶性和与醛改性海藻酸钠 (OSA) 形成凝胶的能力而被选为优化的前体。此外，纤维素纳米晶体 (CNC) 被制备为纳米填料以增强 P( Sarco-GH) -OSA水凝胶。我们证明了共聚物序列和成分含量通过交联密度的变化对形成的水凝胶的性质产生了影响。动态酰基腙键赋予水凝胶pH响应性和可逆网络。封装在水凝胶中的 NIH/3T3 细胞保持了较高的活力和增殖能力，表明纳米复合水凝胶可用于制造定制的响应性药物递送系统或用于组织工程的细胞支架。
更新日期：2019-09-26
中文翻译：
基于共聚肽(o)ides和多糖的原位形成和可逆交联水凝胶
生物医学领域水凝胶的兴起促使它们具有良好的生物相容性、可调节的机械性能和快速的凝胶过程。在此，通过动态酰基腙键构建了一种含有共聚肽(o)ides 和功能性多糖的复合水凝胶。首先，通过肌氨酸 (Sar) 和l-谷氨酸 γ-苄酯 (BLG) N-羧酸酐 (NCA)的开环聚合合成了一系列肽-类肽共聚物。BLG单元的苄基通过酯-酰胺交换氨解反应被酰肼基团取代。聚（肌氨酸-co-谷氨酸-酰肼）的统计共聚物（P（Sar- co-GH)) 因其优异的水溶性和与醛改性海藻酸钠 (OSA) 形成凝胶的能力而被选为优化的前体。此外，纤维素纳米晶体 (CNC) 被制备为纳米填料以增强 P( Sarco-GH) -OSA水凝胶。我们证明了共聚物序列和成分含量通过交联密度的变化对形成的水凝胶的性质产生了影响。动态酰基腙键赋予水凝胶pH响应性和可逆网络。封装在水凝胶中的 NIH/3T3 细胞保持了较高的活力和增殖能力，表明纳米复合水凝胶可用于制造定制的响应性药物递送系统或用于组织工程的细胞支架。
京公网安备 11010802027423号