Yin Yang 1 (YY1) regulates early embryogenesis and adult tissue formation. However, the role of YY1 in stem cell regulation remains unclear. YY1 has a Polycomb group (PcG) protein-dependent role in mammalian cells. The PcG-independent functions of YY1 are also reported, although their underlying mechanism is still undefined. This paper reports the role of YY1 and BAF complex in the OCT4-mediated pluripotency network in mouse embryonic stem cells (mESCs). The interaction between YY1 and BAF complex promotes mESC proliferation and pluripotency. Knockdown of Yy1 or Smarca4, the core component of the BAF complex, downregulates pluripotency markers and upregulates several differentiation markers. Moreover, YY1 enriches at both promoter and super-enhancer regions to stimulate transcription. Thus, this study elucidates the role of YY1 in regulating pluripotency through its interaction with OCT4 and the BAF complex and the role of BAF complex in integrating YY1 into the core pluripotency network.

阴阳1（YY1）调节早期胚胎发生和成人组织形成。然而，YY1在干细胞调节中的作用仍不清楚。YY1在哺乳动物细胞中具有Polycomb group（PcG）蛋白依赖性作用。还报告了YY1的PcG独立功能，尽管其潜在机制仍不确定。本文报道了YY1和BAF复合体在小鼠胚胎干细胞（mESCs）的OCT4介导的多能网络中的作用。YY1和BAF复合物之间的相互作用促进了mESC的增殖和多能性。击倒Yy1或Smarca4BAF复合体的核心成分，下调多能性标志物并上调一些分化标志物。此外，YY1在启动子和超级增强子区域富集以刺激转录。因此，本研究阐明了YY1通过与OCT4和BAF复合物的相互作用来调节多能性的作用，以及BAF复合物在将YY1整合到核心多能性网络中的作用。