Line-scanning confocal microendoscopy offers video-rate cellular imaging of scattering tissue with relatively simple hardware, but its axial response is inferior to that of point-scanning systems. Based on Fourier optics theory, we designed differential confocal apertures with a simple subtraction technique to improve the line-scanning sectioning performance. Taking advantage of digital slit apertures on a digital light projector and a CMOS rolling shutter, we demonstrate real-time optical sectioning performance comparable to point scanning in a dual-camera microendoscope (<$6,000). We validate the background rejection capability when imaging porcine columnar epithelium stained with fluorescent contrast agents with different uptake mechanisms and staining properties.

中文翻译：

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简单的差分数字共焦孔径可改善线扫描共聚焦微内窥镜的轴向响应。

线扫描共聚焦显微内窥镜通过相对简单的硬件就可以对散射组织进行视频速率细胞成像，但是其轴向响应却不如点扫描系统。基于傅立叶光学理论，我们使用简单的减法技术设计了微分共焦孔径，以提高线扫描切片的性能。利用数字投光器和CMOS卷帘上的数字狭缝孔径，我们演示了实时光学切片性能，可与双相机微型内窥镜中的点扫描（不到6,000美元）相提并论。我们验证成像具有不同摄取机制和染色特性的荧光造影剂染色的猪柱状上皮时的背景排斥能力。