模拟紫色非硫细菌中光合作用，CO2固定和醌池之间的相互作用。,Scientific Reports

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模拟紫色非硫细菌中光合作用，CO2固定和醌池之间的相互作用。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-09-02 , DOI: 10.1038/s41598-019-49079-z
Adil Alsiyabi ₁ , Cheryl M Immethun ₁ , Rajib Saha ₁

Affiliation

紫红色假单胞菌CGA009是一种紫色的非硫细菌，可以固定二氧化碳（CO2）和氮，或分解有机化合物以满足其对碳和氮的需求。轻，无机和有机化合物都可以用作其能源。在其代谢过程中产生的过量电子可用于生产氢气或可生物降解的聚酯。重建了细菌的基因组规模代谢模型，以研究光合作用，CO2固定和醌池的氧化还原状态之间的相互作用。将模型预测的通量值与可用的代谢通量分析（MFA）通量进行比较，得出在四种不同的生长基质上的预测误差为5-19％。该模型预测存在一个未确认的沉池，该沉池负责TCA循环产生的过量喹诺酮的氧化。此外，发现依赖光的能量产生高度依赖于喹啉氧化速率。最后，CO2固定的程度预计取决于通过电子传输链产生的ATP的量，过量的ATP进入需要能量的Calvin-Benson-Bassham（CBB）途径。基于此分析，假设醌氧化还原状态充当CBB途径的前馈控制器，表明可用的ATP数量。预计二氧化碳固定的程度取决于通过电子传输链产生的ATP的量，过量的ATP会进入需要能量的Calvin-Benson-Bassham（CBB）途径。基于此分析，假设醌氧化还原状态充当CBB途径的前馈控制器，表明可用的ATP数量。预计二氧化碳固定的程度取决于通过电子传输链产生的ATP的量，过量的ATP会进入需要能量的Calvin-Benson-Bassham（CBB）途径。基于此分析，假设醌氧化还原状态充当CBB途径的前馈控制器，表明可用的ATP数量。

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Modeling the Interplay between Photosynthesis, CO2 Fixation, and the Quinone Pool in a Purple Non-Sulfur Bacterium.

Rhodopseudomonas palustris CGA009 is a purple non-sulfur bacterium that can fix carbon dioxide (CO2) and nitrogen or break down organic compounds for its carbon and nitrogen requirements. Light, inorganic, and organic compounds can all be used for its source of energy. Excess electrons produced during its metabolic processes can be exploited to produce hydrogen gas or biodegradable polyesters. A genome-scale metabolic model of the bacterium was reconstructed to study the interactions between photosynthesis, CO2 fixation, and the redox state of the quinone pool. A comparison of model-predicted flux values with available Metabolic Flux Analysis (MFA) fluxes yielded predicted errors of 5-19% across four different growth substrates. The model predicted the presence of an unidentified sink responsible for the oxidation of excess quinols generated by the TCA cycle. Furthermore, light-dependent energy production was found to be highly dependent on the quinol oxidation rate. Finally, the extent of CO2 fixation was predicted to be dependent on the amount of ATP generated through the electron transport chain, with excess ATP going toward the energy-demanding Calvin-Benson-Bassham (CBB) pathway. Based on this analysis, it is hypothesized that the quinone redox state acts as a feed-forward controller of the CBB pathway, signaling the amount of ATP available.

更新日期：2019-09-03

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