Abstract

In nature, ion channels play key roles in controlling ion transport between cells and their surroundings. Calcium ion (Ca²⁺)-induced Ca²⁺ release (CICR), a critical control mechanism for Ca²⁺ channels, occurs due to a Ca²⁺ concentration gradient working in synergy with ryanodine receptors, which are famously known as “calcium sparks”. Inspired by this self-regulated biological process, a smart Ca²⁺ concentration-modulated nanochannel system was developed by integrating a poly{N-isopropylacrylamide-co-acrylamide-[4-(trifluoromethyl) phenyl]-2-thiourea_0.2-co-acrylamide-DDDEEKC_0.2} (denoted as PNI-co-CF₃-PT_0.2-co-DDDEEKC_0.2) three-component copolymer onto the nanochannels of a porous anodic alumina (PAA) membrane. In this smart polymer design, the DDDEEKC hepta-peptide unit has an extraordinary binding affinity with Ca²⁺ through coordination bonds, while CF₃-PT functions as a hydrogen bond mediation unit, facilitating the remarkable conformational transition of the PNI main chain in response to Ca²⁺-specific adsorption. Due to these futures, the dynamic gating behaviors of the modified nanochannels could be precisely manipulated by the Ca²⁺ concentration. In addition, the sensitive Ca²⁺ response, as low as 10 pM with a high specificity toward Ca²⁺ capable of discriminating Ca²⁺ from other potential interference metal ions (e.g., K⁺, Cu²⁺, Mg²⁺, Zn²⁺, Fe³⁺, and Al³⁺), remarkable morphological change in the nanochannel and satisfactory reversibility indicate the great potential of Ca²⁺-responsive polymers for the fabrication of biodevices and artificial nanochannels.

中文翻译：

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通过模仿生物Ca 2+诱导的Ca 2+释放过程，基于聚合物的智能钙离子自调节纳米通道

抽象的

实际上，离子通道在控制细胞及其周围环境之间的离子迁移中起关键作用。钙离子（Ca ²⁺）诱导的Ca ²⁺释放（CICR），是Ca ²⁺通道的关键控制机制，是由于Ca ²⁺浓度梯度与雷诺丹碱受体协同作用而发生的，众所周知，莱诺丁碱受体被称为“钙”。火花”。通过这种自我调节的生物学过程的启发，智能的Ca ²⁺浓度调制纳米通道系统是由集成一个聚{开发Ñ -isopropylacrylamide-共-acrylamide- [4-（三氟甲基）苯基] -2-硫脲_0.2 -共-丙烯酰胺-DDDEEKC _0.2}（表示为PNI- co -CF ₃ -PT _0.2 - co -DDDEEKC _0.2）三组分共聚物在多孔阳极氧化铝（PAA）膜的纳米通道上。在这种智能聚合物设计中，DDDEEKC七肽单元通过配位键与Ca ²⁺具有非凡的结合亲和力，而CF ₃ -PT则充当氢键介导单元，促进了PNI主链在响应中显着的构象转变对Ca ^2+的特异性吸附。由于这些未来，修饰的纳米通道的动态门控行为可以被Ca ²⁺精确控制。专注。此外，敏感的Ca ²⁺响应低至10 pM，对Ca ²⁺具有高特异性，能够将Ca ²⁺与其他潜在的干扰金属离子（例如K ⁺，Cu 2 ⁺，Mg 2 ⁺，Zn ²⁺，Fe ³⁺和Al ³⁺），纳米通道中的显着形态变化和令人满意的可逆性表明，Ca ²⁺响应聚合物在制造生物装置和人工纳米通道方面具有巨大潜力。