Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.

中文翻译：

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一种高效的生长素诱导型德隆系统，在人类细胞中具有低的基础降解

生长素诱导的degron技术可实现快速而受控的蛋白质耗竭。然而，没有植物生长素的基础降解和植物生长素诱导的无效消耗限制了其实用性。我们已经确定了由植物生长素受体F-box蛋白At AFB2和短地龙miniIAA7组成的有效的生长素诱导型地龙系统。该系统显示出最小的基础降解，并能够在1小时内以强健的功能表型快速去除内源性人类跨膜，细胞质和核蛋白的生长素诱导型消耗。