cDNA Library for Mining Functional Genes in Sedum alfredii Hance Related to Cadmium Tolerance and Characterization of the Roles of a Novel SaCTP2 Gene in Enhancing Cadmium Hyperaccumulation,Environmental Science & Technology

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cDNA Library for Mining Functional Genes in Sedum alfredii Hance Related to Cadmium Tolerance and Characterization of the Roles of a Novel SaCTP2 Gene in Enhancing Cadmium Hyperaccumulation
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2019-09-09 , DOI: 10.1021/acs.est.9b03237
Mingying Liu _{1,

2,

3} , Xuelian He _{1,

2} , Tongyu Feng _{1,

2} , Renying Zhuo _{1,

2} , Wenmin Qiu _{1,

2} , Xiaojiao Han _{1,

2} , Guirong Qiao _{1,

2} , Dayi Zhang ₄

Affiliation

Heavy metal contamination presents serious threats to living organisms. Functional genes related to cadmium (Cd) hypertolerance or hyperaccumulation must be explored to enhance phytoremediation. Sedum alfredii Hance is a Zn/Cd cohyperaccumulator exhibiting abundant genes associated with Cd hypertolerance. Here, we developed a method for screening genes related to Cd tolerance by expressing a cDNA-library for S. alfredii Hance. Yeast functional complementation validated 42 of 48 full-length genes involved in Cd tolerance, and the majority of them were strongly induced in roots and exhibited diverse expression profiles across tissues. Coexpression network analysis suggested that 15 hub genes were connected with genes involved in metabolic processes, response to stimuli, and metal transporter and antioxidant activity. The functions of a novel SaCTP2 gene were validated by heterologous expression in Arabidopsis, responsible for retarding chlorophyll content decrease, maintaining membrane integrity, promoting reactive oxygen species (ROS) scavenger activities, and reducing ROS levels. Our findings suggest a highly complex network of genes related to Cd hypertolerance in S. alfredii Hance, accomplished via the antioxidant system, defense genes induction, and the calcium signaling pathway. The proposed cDNA-library method is an effective approach for mining candidate genes associated with Cd hypertolerance to develop genetically engineered plants for use in phytoremediation.

中文翻译：

采掘与镉耐受性相关的东南景天功能基因的cDNA文库和表征新型SaCTP2基因在增强镉超积累中的作用

重金属污染对生物体构成严重威胁。必须探索与镉（Cd）高耐受性或高积累有关的功能基因，以增强植物修复作用。东南景天（Sedum alfredii Hance）是一种Zn / Cd过度蓄积物，显示出与Cd超耐受性相关的大量基因。在这里，我们开发了一种通过表达苜蓿链球菌的cDNA文库来筛选与镉耐受性相关的基因的方法。昂斯。酵母功能互补验证了涉及Cd耐受性的48个全长基因中的42个，其中大多数在根部强烈诱导，并在组织中表现出不同的表达特征。共表达网络分析表明，有15个中枢基因与参与代谢过程，对刺激的反应以及金属转运蛋白和抗氧化活性的基因相关。一个新的SaCTP2基因的功能已通过在拟南芥中的异源表达进行了验证，该基因负责阻止叶绿素含量的下降，维持膜的完整性，促进活性氧（ROS）清除剂的活性以及降低ROS的水平。我们的发现表明，与S. alfredii中的Cd超耐受性相关的基因的网络非常复杂Hance是通过抗氧化剂系统，防御基因诱导和钙信号传导途径完成的。拟议的cDNA文库方法是一种有效的方法，用于挖掘与镉超耐受性相关的候选基因，以开发用于植物修复的基因工程植物。

更新日期：2019-09-09

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