Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization.,Microbial Biotechnology

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Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization.
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2019-08-26 , DOI: 10.1111/1751-7915.13478
Zhiqiang Wen ₁ , Qi Li ₂ , Jinle Liu ₃ , Mingjie Jin ₁ , Sheng Yang _{3,

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Affiliation

Butanol is an important bulk chemical, as well as a promising renewable gasoline substitute, that is commonly produced by solventogenic Clostridia. The main cost of cellulosic butanol fermentation is caused by cellulases that are required to saccharify lignocellulose, since solventogenic Clostridia cannot efficiently secrete cellulases. However, cellulolytic Clostridia can natively degrade lignocellulose and produce ethanol, acetate, butyrate and even butanol. Therefore, cellulolytic Clostridia offer an alternative to develop consolidated bioprocessing (CBP), which combines cellulase production, lignocellulose hydrolysis and co‐fermentation of hexose/pentose into butanol in one step. This review focuses on CBP advances for butanol production of cellulolytic Clostridia and various synthetic biotechnologies that drive these advances. Moreover, the efforts to optimize the CBP‐enabling cellulolytic Clostridia chassis are also discussed. These include the development of genetic tools, pentose metabolic engineering and the improvement of butanol tolerance. Designer cellulolytic Clostridia or consortium provide a promising approach and resource to accelerate future CBP for butanol production.

中文翻译：

纤维素分解梭菌丁醇生产的综合生物工艺：开发和优化。

丁醇是一种重要的大宗化学品，也是一种有前途的可再生汽油替代品，通常由产溶剂梭菌生产。纤维素丁醇发酵的主要成本是由糖化木质纤维素所需的纤维素酶引起的，因为产溶剂梭菌不能有效地分泌纤维素酶。然而，纤维素分解梭菌可以天然降解木质纤维素并产生乙醇、乙酸盐、丁酸盐甚至丁醇。因此，纤维素分解梭状芽胞杆菌为开发综合生物加工（CBP）提供了一种替代方案，它将纤维素酶生产、木质纤维素水解和己糖/戊糖共发酵为丁醇一步结合在一起。本综述重点关注 CBP 在纤维素分解梭菌丁醇生产方面的进展以及推动这些进展的各种合成生物技术。此外，还讨论了优化支持 CBP 的纤维素分解梭菌底盘的努力。其中包括遗传工具的开发、戊糖代谢工程和丁醇耐受性的改善。设计师纤维素分解梭菌或联盟提供了一种有前途的方法和资源，以加速未来丁醇生产的 CBP。

更新日期：2019-08-26

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