Signal transduction is driven by protein interactions that are controlled by posttranslational modifications (PTM). Usually, protein domains are responsible for "reading" the PTM signal deposited on the interacting partners. Protein domain microarrays have been developed as a high throughput platform to facilitate the rapid identification of protein-protein interactions, and this approach has become broadly used in biomedical research. In this review, we will summarize the history, development and applications of this technique, including the use of protein domain microarrays in identifying both novel protein-protein interactions and small molecules that block these interactions. We will focus on the approaches we use in the Protein Array and Analysis Core - the PAAC - at MD Anderson Cancer Center. We will also address the technical limitations and discuss future directions. (125 words).

中文翻译：

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蛋白质域微阵列作为破译信号通路和组蛋白代码的平台

信号转导由受翻译后修饰 (PTM) 控制的蛋白质相互作用驱动。通常，蛋白质域负责“读取”沉积在相互作用伙伴上的 PTM 信号。蛋白质域微阵列已被开发为一种高通量平台，以促进蛋白质-蛋白质相互作用的快速识别，这种方法已广泛用于生物医学研究。在这篇综述中，我们将总结这项技术的历史、发展和应用，包括使用蛋白质域微阵列来识别新的蛋白质 - 蛋白质相互作用和阻止这些相互作用的小分子。我们将重点介绍我们在 MD 安德森癌症中心的蛋白质阵列和分析核心 - PAAC 中使用的方法。我们还将解决技术限制并讨论未来的方向。（125 字）。