GABAa受体亚基在银cat鱼（Rhamdia quelen）脑中的表达及其受Nectandra grandiflora Nees精油和分离的化合物的调节。,Behavioural Brain Research

当前位置： X-MOL 学术 › Behav. Brain Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

GABAa受体亚基在银cat鱼（Rhamdia quelen）脑中的表达及其受Nectandra grandiflora Nees精油和分离的化合物的调节。
Behavioural Brain Research ( IF 2.6 ) Pub Date : 2019-08-24 , DOI: 10.1016/j.bbr.2019.112178
Quelen I Garlet ₁ , Carine F Souza ₁ , Patrícia Rodrigues ₁ , Sharine N Descovi ₂ , Gonzalo Martinez-Rodríguez ₃ , Bernardo Baldisserotto ₁ , Berta M Heinzmann ₁

Affiliation

使用银cat鱼（Rhamdia quelen）作为实验模型的研究通常用于筛选具有GABA能介导作用的精油（EO）。然而，银cat鱼脑中GABA a受体的表达仍是未知的。因此，我们评估了银fish鱼是否表达与镇静/麻醉过程和/或神经系统疾病相关的GABA a受体亚基。此外，我们评估了用大花蜜（Nectandra grandiflora EO）及其分离的化合物，鱼的麻醉药（+）-脱氢富马酮（DHF）和脱氢富马酮环氧化合物（DFX）镇静的鱼中GABAa受体亚基的脑表达，eremophil-11-en-10- ol（ERM）和selin-11-en-4-α-ol（SEL），它们在小鼠中具有GABAa介导的抗焦虑样作用。gabra1，gabra2，gabra3，gabrb1，通过实时PCR在24h镇静浴后评估银silver鱼脑中的gabrd和gabrg2。由于qPCR数据很少描述作用机理，通常通过与受体的相互作用来发现作用机理，因此我们还使用氟马西尼（FMZ）进行了拮抗剂驱动的实验。实时荧光定量PCR检测了古兰经脑中所有靶基因的mRNA表达。在用ERM镇静的鱼中gabra1的表达降低；EO增加gabra2，gabra3，gabrb1和gabrg2表达；SEL增加了gabrb1，gabrd和gabrg2的表达。EO和DFX，SEL和ERM化合物诱导鱼的持续镇静作用，而FMZ浴则促使ERM和DFX诱导的镇静作用恢复。我们的结果表明，EO，SEL，ERM和DFX的镇静作用涉及与GABA能系统的相互作用。

"点击查看英文标题和摘要"

GABAa receptor subunits expression in silver catfish (Rhamdia quelen) brain and its modulation by Nectandra grandiflora Nees essential oil and isolated compounds.

Studies using silver catfish (Rhamdia quelen) as experimental models are often applied to screen essential oils (EO) with GABAergic-mediated effects. However, the expression of GABAa receptors in the silver catfish brain remains unknown. Thus, we assessed whether silver catfish express GABAa receptor subunits associated with sedation/anesthetic process and/or neurological diseases. Additionally, we evaluated the brain expression of GABAa receptor subunits in fish sedated with Nectandra grandiflora EO and its isolated compounds, the fish anesthetic (+)-dehydrofukinone (DHF), and dehydrofukinone epoxide (DFX), eremophil-11-en-10-ol (ERM) and selin-11-en-4-α-ol (SEL), which have GABAa-mediated anxiolytic-like effects in mice. The expression of the subunits gabra1, gabra2, gabra3, gabrb1, gabrd and gabrg2 in the silver catfish brain were assessed after a 24h-sedation bath by real time PCR. Since qPCR data rarely describes mechanisms of action, which are usually found through interactions with receptors, we also performed an antagonist-driven experiment using flumazenil (FMZ). Real-time PCR detected the mRNA expression of all targeted genes in R. quelen brain. The expression of gabra1 was decreased in fish sedated with ERM; EO increased gabra2, gabra3, gabrb1 and gabrg2 expression; SEL increased gabrb1, gabrd and gabrg2 expression. EO and compounds DFX, SEL and ERM induced sustained sedation in fish and FMZ-bath prompted the recovery from ERM- and DFX-induced sedation. Our results suggest that the EO, SEL, ERM and DFX sedative effects involve interaction with the GABAergic system. Our findings support the use of the silver catfish as robust and reliable experimental model to evaluate the efficacy of drugs with putative GABAergic-mediated effects.

更新日期：2019-08-24

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南