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Detection of microRNAs using toehold-initiated rolling circle amplification and fluorescence resonance energy transfer.
Talanta ( IF 5.6 ) Pub Date : 2019-08-24 , DOI: 10.1016/j.talanta.2019.120285
Kaili Liang 1 , Hui Wang 1 , Ping Li 1 , Youming Zhu 1 , Ju Liu 2 , Bo Tang 1
Talanta ( IF 5.6 ) Pub Date : 2019-08-24 , DOI: 10.1016/j.talanta.2019.120285
Kaili Liang 1 , Hui Wang 1 , Ping Li 1 , Youming Zhu 1 , Ju Liu 2 , Bo Tang 1
Affiliation
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Detection of microRNAs (miRNAs) in cells improves our understanding of their physiological functions and facilitates exploration of their roles diseases. The toehold-mediated strand displacement reaction initiates rolling circle amplification (RCA) to achieve signal amplification of the specific miRNA; This process is named as toehold-initiated RCA (TIRCA). The product of TIRCA was ligated to two DNA probes, which were modified with 6-carboxyfluorescein and carboxytetramethylrhodamine, respectively. Qualitative detection of miRNAs was successfully achieved by combining the fluorescence aggregation enhancement effect with fluorescence resonance energy transfer generated by the proximity of the two fluorescent dyes. Thus, this approach helps us analyze the roles of miRNAs in human disease more accurately.
中文翻译：
使用脚趾引发的滚环扩增和荧光共振能量转移检测microRNA。
细胞中微小RNA（miRNA）的检测可增进我们对它们的生理功能的了解，并有助于探索其所扮演的疾病。脚趾介导的链置换反应启动滚环扩增（RCA），以实现特定miRNA的信号扩增。此过程称为由Toehold发起的RCA（TIRCA）。将TIRCA的产物连接到两个DNA探针上，分别用6-羧基荧光素和羧基四甲基罗丹明修饰。通过将荧光聚集增强作用与两种荧光染料附近产生的荧光共振能量转移相结合，成功实现了miRNA的定性检测。因此，这种方法有助于我们更准确地分析miRNA在人类疾病中的作用。
更新日期：2019-08-24
中文翻译：
使用脚趾引发的滚环扩增和荧光共振能量转移检测microRNA。
细胞中微小RNA（miRNA）的检测可增进我们对它们的生理功能的了解，并有助于探索其所扮演的疾病。脚趾介导的链置换反应启动滚环扩增（RCA），以实现特定miRNA的信号扩增。此过程称为由Toehold发起的RCA（TIRCA）。将TIRCA的产物连接到两个DNA探针上，分别用6-羧基荧光素和羧基四甲基罗丹明修饰。通过将荧光聚集增强作用与两种荧光染料附近产生的荧光共振能量转移相结合，成功实现了miRNA的定性检测。因此，这种方法有助于我们更准确地分析miRNA在人类疾病中的作用。
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