It is challenge for the stable operation of the anaerobic ammonium oxidation (Anammox) based wastewater treatment processes. The feasibility for the startup of the partial nitritation and Anammox (PNA) process was demonstrated with cascade oxygen supply by controlling anoxic/aerobic durations. Under steady state conditions, total nitrogen removal percentages of 63.2% (pulse feeding) and 88.0% (constant feeding) were obtained with the anoxic/aerobic duration of 5 min/7 min at 26 °C, and PNA was successfully achieved. The microbial community analysis revealed that Candidatus Kuenenia and Nitrosomonas were dominant genera of Anammox bacteria and ammonia oxidizing bacteria, respectively. Microbial interactions were examined through acyl-homoserine lactones-based quorum sensing (AHLs-QS) and metagenomics analyses. N-octanoyl-homoserine lactone, N-decanoyl homoserine lactone and N-dodecanoyl homoserine lactone had obvious relationships with the abundance of the Anammox bacteria. The AHLs-QS system could control microbial interactions among Anammox bacteria, nitrifiers and heterotrophs, especially for their balances in the PNA system.

对于基于厌氧铵氧化（Anammox）的废水处理工艺的稳定运行而言，这是一个挑战。通过控制缺氧/好氧持续时间，通过级联供氧证明了启动部分亚硝化和厌氧氨氧化（PNA）工艺的可行性。在稳态条件下，在26°C下缺氧/有氧持续时间为5分钟/ 7分钟时，总脱氮率分别为63.2％（脉冲进料）和88.0％（恒定进料），并且成功实现了PNA。微生物群落分析表明，暂定Kuenenia和亚硝化分别是厌氧氨氧化细菌和氨氧化细菌的优势属。通过基于酰基高丝氨酸内酯的群体感应（AHLs-QS）和宏基因组学分析检查了微生物之间的相互作用。N-辛酰基-高丝氨酸内酯，N-癸酰基高丝氨酸内酯和N-十二烷酰基高丝氨酸内酯与厌氧细菌的丰度有着明显的关系。AHLs-QS系统可以控制Anammox细菌，硝化剂和异养菌之间的微生物相互作用，尤其是它们在PNA系统中的平衡。