An Efficient 1064 nm NIR‐II Excitation Fluorescent Molecular Dye for Deep‐Tissue High‐Resolution Dynamic Bioimaging,Angewandte Chemie International Edition

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An Efficient 1064 nm NIR‐II Excitation Fluorescent Molecular Dye for Deep‐Tissue High‐Resolution Dynamic Bioimaging
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2018-03-24 , DOI: 10.1002/anie.201801226
Benhao Li ₁ , Lingfei Lu ₁ , Mengyao Zhao ₁ , Zuhai Lei ₁ , Fan Zhang ₁

Affiliation

A small‐molecule fluorophore FD‐1080 with both excitation and emission in the NIR‐II region has been successfully synthesized for in vivo imaging. A heptamethine structure is designed to shift the absorption and emission into NIR‐II region. Sulphonic and cyclohexene groups are introduced to enhance its water solubility and stability. The quantum yield of FD‐1080 is 0.31 %, and can be increased to 5.94 % after combining with fetal bovine serum (FBS). Significantly, 1064 nm NIR‐II excitation was demonstrated with the high tissue penetration depth and superior imaging resolution compared to previously reported NIR excitation from 650 nm to 980 nm. FD‐1080 is not only capable of realizing non‐invasive high‐resolution deep‐tissue hindlimb vasculature and brain vessel bioimaging, but also quantifying the respiratory rate based on the dynamic imaging of respiratory craniocaudal motion of the liver for the awake and anaesthetized mouse.

中文翻译：

用于深层组织高分辨率动态生物成像的高效1064 nm NIR-II激发荧光分子染料

已经成功合成了在NIR-II区域具有激发和发射作用的小分子荧光团FD-1080，用于体内成像。七甲胺的结构旨在将吸收和发射转移到NIR-II区。引入磺基和环己烯基团以增强其水溶性和稳定性。FD‐1080的量子产率为0.31％，与胎牛血清（FBS）结合后可提高至5.94％。值得注意的是，与先前报道的从650 nm到980 nm的NIR激发相比，证明了1064 nm NIR-II激发具有较高的组织穿透深度和优异的成像分辨率。FD‐1080不仅能够实现无创的高分辨率深层组织后肢血管系统和脑血管生物成像，

更新日期：2018-03-24

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