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A highly sensitive tetracycline sensor based on a combination of magnetic molecularly imprinted polymer nanoparticles and surface plasmon resonance detection
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-08-22 , DOI: 10.1007/s00604-019-3718-9
Wanru Gao , Pao Li , Si Qin , Zhao Huang , Yanan Cao , Xia Liu

A method is reported for in-situ detection of the antibiotic tetracycline (TC). It is based on a combination of extraction of TC by magnetic molecularly imprinted polymers nanoparticles (MMIPs NPs) and detection by surface plasmon resonance (SPR). The TC-captured MMIPs NPs were flowed over the surface of the SPR chip that was modified with mercaptoethylamine. The SPR signal undergoes a strong increase through the use of MMIPs NPs. It increases linearly in the 5.0–100 pg·mL−1 TC concentration range, and the detection limit is as low as 1.0 pg·mL−1 (at S/N = 3). This method shows selectivity to TC compared with structurally analogues. In order to demonstrate the power of the method, it was applied to the analysis of milk spiked with TC. These results were validated by comparing them to those of an enzyme-linked immunoassay. The average recovery is in the range of 95.7–104.6%. Graphical abstract Schematic representation of a surface plasmon resonance assay for the sensitive determination of tetracycline in milk using the magnetic molecularly imprinted polymers nanoparticles that can extract tetracycline and amplify the SPR signal. Schematic representation of a surface plasmon resonance assay for the sensitive determination of tetracycline in milk using the magnetic molecularly imprinted polymers nanoparticles that can extract tetracycline and amplify the SPR signal.

中文翻译:

基于磁性分子印迹聚合物纳米粒子和表面等离子体共振检测组合的高灵敏度四环素传感器

报道了一种原位检测抗生素四环素 (TC) 的方法。它基于通过磁性分子印迹聚合物纳米粒子 (MMIPs NPs) 提取 TC 和通过表面等离子体共振 (SPR) 检测的组合。TC 捕获的 MMIPs NPs 流过用巯基乙胺修饰的 SPR 芯片的表面。通过使用 MMIPs NPs,SPR 信号经历了强烈的增加。它在 5.0–100 pg·mL-1 TC 浓度范围内线性增加,检测限低至 1.0 pg·mL-1(S/N = 3)。与结构类似物相比,该方法显示出对 TC 的选择性。为了证明该方法的有效性,将其应用于添加 TC 的牛奶的分析。通过将这些结果与酶联免疫测定的结果进行比较来验证这些结果。平均回收率在 95.7-104.6% 的范围内。图形摘要 使用可提取四环素并放大 SPR 信号的磁性分子印迹聚合物纳米颗粒,用于灵敏测定牛奶中四环素的表面等离子共振测定的示意图。使用可提取四环素并放大 SPR 信号的磁性分子印迹聚合物纳米颗粒,用于灵敏测定牛奶中四环素的表面等离子共振测定的示意图。
更新日期:2019-08-22
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