使用BODIPY分子探针在体外和细胞中感测温度。,The Journal of Physical Chemistry B

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使用BODIPY分子探针在体外和细胞中感测温度。
The Journal of Physical Chemistry B ( IF 2.8 ) Pub Date : 2019-08-20 , DOI: 10.1021/acs.jpcb.9b04384
Meredith M Ogle , Ashleigh D Smith McWilliams , Matthew J Ware ₁ , Steven A Curley _{1,

2} , Stuart J Corr _{1,

3,

4} , Angel A Martí

Affiliation

硼二吡咯亚甲基（BODIPY）分子转子已显示出对粘度，极性和温度的敏感性。在这里，我们报告一个1,3,5,7-四甲基-8-苯基-BODIPY修饰的聚乙二醇（PEG）链，用于温度感测和活细胞成像。这种新的PEG-BODIPY染料随着温度的升高而呈现出非辐射衰减的增加，这直接影响了其寿命。寿命的这种变化取决于在低粘度值下温度和粘度的变化，但是仅取决于在高粘度值下的温度。荧光寿命与温度的相关性允许以亚度分辨率在体外和细胞内进行温度监测。与细胞接触时，PEG-BODIPY自发渗透并染色细胞，但不染色细胞核。此外，即使在100μM的浓度下也没有发现明显的细胞毒性。使用荧光寿命成像显微镜（FLIM），我们能够观察到不同温度下细胞内PEG-BODIPY寿命的变化。使用FLIM和分子探针（例如PEG-BODIPY）可以在医学上相关的刺激（例如射频消融和光动力疗法）后提供有关细胞温度和散热的重要信息。

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Sensing Temperature in Vitro and in Cells Using a BODIPY Molecular Probe.

Boron dipyrromethene (BODIPY) molecular rotors have shown sensitivity toward viscosity, polarity, and temperature. Here, we report a 1,3,5,7-tetramethyl-8-phenyl-BODIPY modified with a polyethylene glycol (PEG) chain, for temperature sensing and live cell imaging. This new PEG-BODIPY dye presents an increase in nonradiative decay as temperature increases, which directly influences its lifetime. This change in lifetime is dependent on changes in both temperature and viscosity at low viscosity values, but is only dependent on temperature at high viscosity values. The dependence of fluorescence lifetime with temperature allows for temperature monitoring in vitro and in cells, with sub degree resolution. When in contact with cells, the PEG-BODIPY spontaneously penetrates and stains the cell but not the nucleus. Furthermore, no significant cell toxicity was found even at 100 μM concentration. Using fluorescence lifetime imaging microscopy (FLIM), we were able to observe the changes in the lifetime of PEG-BODIPY within the cell at different temperatures. The use of FLIM and molecular probes such as PEG-BODIPY can provide important information about cellular temperature and heat dissipation upon medically relevant stimuli, such as radiofrequency ablation and photodynamic therapy.

更新日期：2019-08-20

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