MICU1 controls cristae junction and spatially anchors mitochondrial Ca2+ uniporter complex.,Nature Communications

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MICU1 controls cristae junction and spatially anchors mitochondrial Ca2+ uniporter complex.
Nature Communications ( IF 14.7 ) Pub Date : 2019-08-19 , DOI: 10.1038/s41467-019-11692-x
Benjamin Gottschalk ₁ , Christiane Klec ₁ , Gerd Leitinger ₂ , Eva Bernhart ₁ , René Rost ₁ , Helmut Bischof ₁ , Corina T Madreiter-Sokolowski ₁ , Snježana Radulović _{1,

2} , Emrah Eroglu ₁ , Wolfgang Sattler _{1,

3} , Markus Waldeck-Weiermair ₁ , Roland Malli _{1,

3} , Wolfgang F Graier _{1,

3}

Affiliation

Recently identified core proteins (MICU1, MCU, EMRE) forming the mitochondrial Ca2+ uniporter complex propelled investigations into its physiological workings. Here, we apply structured illumination microscopy to visualize and localize these proteins in living cells. Our data show that MICU1 localizes at the inner boundary membrane (IBM) due to electrostatic interaction of its polybasic domain. Moreover, this exclusive localization of MICU1 is important for the stability of cristae junctions (CJ), cytochrome c release and mitochondrial membrane potential. In contrast to MICU1, MCU and EMRE are homogeneously distributed at the inner mitochondrial membrane under resting conditions. However, upon Ca2+ elevation MCU and EMRE dynamically accumulate at the IBM in a MICU1-dependent manner. Eventually, our findings unveil an essential function of MICU1 in CJ stabilization and provide mechanistic insights of how sophistically MICU1 controls the MCU-Complex while maintaining the structural mitochondrial membrane framework.

中文翻译：

MICU1 控制嵴连接并在空间上锚定线粒体 Ca2+ 单向转运体复合体。

最近发现的核心蛋白（MICU1、MCU、EMRE）形成线粒体 Ca2+ 单向转运体复合体，推动了对其生理工作的研究。在这里，我们应用结构化照明显微镜来可视化和定位活细胞中的这些蛋白质。我们的数据显示 MICU1 由于其多元结构域的静电相互作用而定位于内边界膜 (IBM)。此外，MICU1 的这种独特定位对于嵴连接 (CJ) 的稳定性、细胞色素 c 释放和线粒体膜电位非常重要。与 MICU1 相比，MCU 和 EMRE 在静息条件下均匀分布在内线粒体膜上。然而，在 Ca2+ 升高时，MCU 和 EMRE 以依赖于 MICU1 的方式在 IBM 动态积累。最终，

更新日期：2019-08-19

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