从沙棘叶中提取多酚的半连续工艺。,Scientific Reports

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从沙棘叶中提取多酚的半连续工艺。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-08-19 , DOI: 10.1038/s41598-019-48610-6
Ioana Asofiei ₁ , Ioan Calinescu ₁ , Adrian Trifan ₁ , Adina Ionuta Gavrila ₁

Affiliation

沙棘 (Hippophae Rhamnoides L.) 是生物活性化合物的重要来源，例如：抗氧化剂、维生素、脂肪酸、氨基酸和矿物质。沙棘叶提取物已被证明具有抗微生物、抗氧化、抗炎和抗病毒特性。本文描述了使用半连续小型反应器从沙棘叶中提取多酚。提取条件不得影响多酚的组成和结构。为此，研究了不同参数（停留时间、溶剂流速、搅拌速率、反应器类型和溶剂预热）对萃取过程的影响。对多酚提取物进行分析，以确定总酚含量 (TPC)、抗氧化能力和主要酚类化合物的浓度。TPC 随着搅拌速率的增加而增加。预热溶剂可提高产率并缩短停留时间。抗氧化能力与TPC结果一致。HPLC 分析证实半连续微波辅助提取 (MAE) 不会影响提取物的成分。与传统萃取 (CE) 相比，MAE 的能耗显着降低。

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A Semi-Continuous Process For Polyphenols Extraction From Sea Buckthorn Leaves.

Sea buckthorn (Hippophae Rhamnoides L.) is an important source of bioactive compounds such as: antioxidants, vitamins, fatty acids, amino acids, and minerals. Sea buckthorn leaves extracts have been proved to have anti-microbial, antioxidant, anti-inflammatory, and anti-viral properties. In this paper, the extraction of polyphenols from sea buckthorn leaves using a semi-continuous small-scale reactor is described. The extraction conditions must not affect the composition and structure of polyphenols. For this reason, the influence of different parameters (residence time, solvent flow rate, stirring rate, reactor type, and solvent pre-heating) on the extraction process were studied. The polyphenolic extracts were analyzed in order to determine the total phenolic content (TPC), the antioxidant capacity and the concentration of the main phenolic compounds. The TPC increases with the stirring rate. Pre-heating the solvent leads to a better yield and reduced residence time. The antioxidant capacity is in concordance with the TPC results. HPLC analysis confirms that the semi-continuous microwave assisted extraction (MAE) does not affect the composition of the extracts. The energy consumption was significantly lower for MAE compared with conventional extraction (CE).

更新日期：2019-08-19

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