当前位置：
X-MOL 学术
›
Anal. Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Signal-Off Electrogenerated Chemiluminescence Biosensing Platform Based on the Quenching Effect between Ferrocene and Ru(bpy)32+-Functionalized Metal–Organic Frameworks for the Detection of Methylated RNA
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-08-15 00:00:00 , DOI: 10.1021/acs.analchem.9b02569 Wanqiao Bai 1 , Aiping Cui 1 , Meizhou Liu 1 , Xuezhi Qiao 2 , Yan Li 1 , Tie Wang 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2019-08-15 00:00:00 , DOI: 10.1021/acs.analchem.9b02569 Wanqiao Bai 1 , Aiping Cui 1 , Meizhou Liu 1 , Xuezhi Qiao 2 , Yan Li 1 , Tie Wang 2
Affiliation
|
N6-methyladenine (m6A), one of the most common chemical modifications of eukaryotic RNA, participates in many important biological processes. An effective strategy for the quantitative determination of m6A is of great significance. Herein, we used methylated microRNA-21 (miRNA21) as the model target to propose a simple and sensitive electrogenerated chemiluminescence (ECL) biosensing platform to detect a specific m6A RNA sequence. This strategy is based on the fact that the anti-m6A-antibody can specifically recognize and bind to the m6A site in the RNA sequence, resulting in a quenching effect between Ru(bpy)32+-functionalized metal–organic frameworks and ferrocene. Luminescent metal–organic frameworks ([email protected]) not only act as ECL indicators but also serve as nanoreactors for the relative ECL reactions owing to their porous or multichannel structure, which overcomes the fact that Ru(bpy)32+ is easily released when used for aqueous-phase detection, thus enhancing the ECL efficiency. Moreover, the ECL method has fewer modification steps and uses only one antibody to recognize the target RNA sequence, which simplifies the operation process and reduces the detection time, presenting a wide linear range (0.001–10 nM) for m6A RNA determination with a low detection limit (0.0003 nM). Additionally, this developed strategy was validated for m6A RNA detection in human serum. Thus, the ECL biosensing method provides a new method for m6A RNA determination that is simple, highly specific, and sensitive.
中文翻译：
基于二茂铁与Ru（bpy）3 2+的猝灭效应的无信号电生化学发光生物传感平台-用于检测甲基化RNA的功能化金属有机框架
N 6-甲基腺嘌呤（m 6 A）是真核RNA最常见的化学修饰之一，它参与许多重要的生物学过程。定量测定m 6 A的有效策略具有重要意义。本文中，我们使用甲基化的microRNA-21（miRNA21）作为模型目标，提出了一种简单而灵敏的电化学发光（ECL）生物传感平台来检测特定的m 6 A RNA序列。此策略基于以下事实：抗m 6 A抗体可以特异性识别并结合RNA序列中的m 6 A位点，从而导致Ru（bpy）3 2+之间的猝灭作用功能化的金属有机骨架和二茂铁。发光的金属有机框架（[受电子邮件保护]）不仅充当ECL指示剂，而且由于其多孔或多通道结构，还可以作为相对ECL反应的纳米反应器，从而克服了Ru（bpy）3 2+易于释放的事实当用于水相检测时，从而提高了ECL效率。此外，ECL方法具有更少的修饰步骤，并且仅使用一种抗体即可识别目标RNA序列，从而简化了操作过程并缩短了检测时间，从而为m 6 A RNA测定提供了宽广的线性范围（0.001–10 nM）。低检测限（0.0003 nM）。此外，该开发的策略在m 6时得到了验证人血清中的RNA检测。因此，ECL生物传感方法为m 6 A RNA的测定提供了一种简单，高度特异性和灵敏的新方法。
更新日期：2019-08-15
中文翻译：
基于二茂铁与Ru（bpy）3 2+的猝灭效应的无信号电生化学发光生物传感平台-用于检测甲基化RNA的功能化金属有机框架
N 6-甲基腺嘌呤（m 6 A）是真核RNA最常见的化学修饰之一，它参与许多重要的生物学过程。定量测定m 6 A的有效策略具有重要意义。本文中，我们使用甲基化的microRNA-21（miRNA21）作为模型目标，提出了一种简单而灵敏的电化学发光（ECL）生物传感平台来检测特定的m 6 A RNA序列。此策略基于以下事实：抗m 6 A抗体可以特异性识别并结合RNA序列中的m 6 A位点，从而导致Ru（bpy）3 2+之间的猝灭作用功能化的金属有机骨架和二茂铁。发光的金属有机框架（[受电子邮件保护]）不仅充当ECL指示剂，而且由于其多孔或多通道结构，还可以作为相对ECL反应的纳米反应器，从而克服了Ru（bpy）3 2+易于释放的事实当用于水相检测时，从而提高了ECL效率。此外，ECL方法具有更少的修饰步骤，并且仅使用一种抗体即可识别目标RNA序列，从而简化了操作过程并缩短了检测时间，从而为m 6 A RNA测定提供了宽广的线性范围（0.001–10 nM）。低检测限（0.0003 nM）。此外，该开发的策略在m 6时得到了验证人血清中的RNA检测。因此，ECL生物传感方法为m 6 A RNA的测定提供了一种简单，高度特异性和灵敏的新方法。
京公网安备 11010802027423号