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Hydrogel-Coated Microneedle Arrays for Minimally Invasive Sampling and Sensing of Specific Circulating Nucleic Acids from Skin Interstitial Fluid.
ACS Nano ( IF 15.8 ) Pub Date : 2019-08-14 00:00:00 , DOI: 10.1021/acsnano.9b04783 Dana Al Sulaiman 1 , Jason Y H Chang , Nitasha R Bennett , Helena Topouzi 1 , Claire A Higgins 1 , Darrell J Irvine 2, 3 , Sylvain Ladame 1
ACS Nano ( IF 15.8 ) Pub Date : 2019-08-14 00:00:00 , DOI: 10.1021/acsnano.9b04783 Dana Al Sulaiman 1 , Jason Y H Chang , Nitasha R Bennett , Helena Topouzi 1 , Claire A Higgins 1 , Darrell J Irvine 2, 3 , Sylvain Ladame 1
Affiliation
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Minimally invasive technologies that can sample and detect cell-free nucleic acid biomarkers from liquid biopsies have recently emerged as clinically useful for early diagnosis of a broad range of pathologies, including cancer. Although blood has so far been the most commonly interrogated bodily fluid, skin interstitial fluid has been mostly overlooked despite containing the same broad variety of molecular biomarkers originating from cells and surrounding blood capillaries. Emerging technologies to sample this fluid in a pain-free and minimally-invasive manner often take the form of microneedle patches. Herein, we developed microneedles that are coated with an alginate–peptide nucleic acid hybrid material for sequence-specific sampling, isolation, and detection of nucleic acid biomarkers from skin interstitial fluid. Characterized by fast sampling kinetics and large sampling capacity (∼6.5 μL in 2 min), this platform technology also enables the detection of specific nucleic acid biomarkers either on the patch itself or in solution after light-triggered release from the hydrogel. Considering the emergence of cell-free nucleic acids in bodily fluids as clinically informative biomarkers, platform technologies that can detect them in an automated and minimally invasive fashion have great potential for personalized diagnosis and longitudinal monitoring of patient-specific disease progression.
中文翻译：
水凝胶涂层微针阵列，用于微创采样和感测皮肤间质液中的特定循环核酸。
最近出现的微创技术可以从液体活检中取样和检测无细胞核酸生物标志物，在临床上可用于包括癌症在内的广泛病理学的早期诊断。尽管血液迄今为止是最常被研究的体液，但皮肤间质液尽管含有同样广泛的源自细胞和周围毛细血管的分子生物标志物，但大多被忽视。以无痛和微创方式对这种液体进行采样的新兴技术通常采用微针贴片的形式。在此，我们开发了涂有藻酸盐-肽核酸混合材料的微针，用于对皮肤间质液中的核酸生物标志物进行序列特异性采样、分离和检测。该平台技术具有快速采样动力学和大采样容量（2 分钟内约 6.5 μL）的特点，还能够在光触发从水凝胶中释放后检测贴片本身或溶液中的特定核酸生物标志物。考虑到体液中无细胞核酸作为临床信息生物标志物的出现，能够以自动化和微创方式检测它们的平台技术在个性化诊断和纵向监测患者特定疾病进展方面具有巨大潜力。
更新日期：2019-08-14
中文翻译：
水凝胶涂层微针阵列，用于微创采样和感测皮肤间质液中的特定循环核酸。
最近出现的微创技术可以从液体活检中取样和检测无细胞核酸生物标志物，在临床上可用于包括癌症在内的广泛病理学的早期诊断。尽管血液迄今为止是最常被研究的体液，但皮肤间质液尽管含有同样广泛的源自细胞和周围毛细血管的分子生物标志物，但大多被忽视。以无痛和微创方式对这种液体进行采样的新兴技术通常采用微针贴片的形式。在此，我们开发了涂有藻酸盐-肽核酸混合材料的微针，用于对皮肤间质液中的核酸生物标志物进行序列特异性采样、分离和检测。该平台技术具有快速采样动力学和大采样容量（2 分钟内约 6.5 μL）的特点，还能够在光触发从水凝胶中释放后检测贴片本身或溶液中的特定核酸生物标志物。考虑到体液中无细胞核酸作为临床信息生物标志物的出现，能够以自动化和微创方式检测它们的平台技术在个性化诊断和纵向监测患者特定疾病进展方面具有巨大潜力。
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