可排泄的 IR-820 用于体内 NIR-II 荧光脑血管成像和皮下肿瘤的光热治疗。,Theranostics

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可排泄的 IR-820 用于体内 NIR-II 荧光脑血管成像和皮下肿瘤的光热治疗。
Theranostics ( IF 12.4 ) Pub Date : 2019-08-09 , DOI: 10.7150/thno.31332
Zhe Feng ₁ , Xiaoming Yu ₂ , Minxiao Jiang ₂ , Liang Zhu ₃ , Yi Zhang ₄ , Wei Yang ₄ , Wang Xi ₃ , Gonghui Li ₂ , Jun Qian _{1,

2}

Affiliation

理由：脑血管疾病与恶性肿瘤一起，当今仍然对人类健康构成巨大威胁。凭借其高空间分辨率和大穿透深度的优势，第二近红外光谱区（NIR-II，900-1700 nm）的荧光生物成像及其基于生物相容性荧光染料的相关成像引导治疗已成为一种有前途的治疗诊断学。方法。方法：通过长期观察验证我们在NIR-II荧光生物成像中使用的IR-820的生物相容性。建立小鼠颅窗模型、小鼠大脑中动脉闭塞模型、小鼠膀胱肿瘤皮下移植模型。采用IR-820辅助NIR-II荧光显微镜进行NIR-II荧光脑血管功能成像。膀胱肿瘤采用 NIR-II 荧光成像引导光热疗法治疗。结果：我们发现 IR-820 具有相当大的 NIR-II 荧光强度，并且在血清中比在水中显示出更高的亮度。在此，我们基于IR-820辅助NIR-II荧光显微镜，实现了高空间分辨率和大穿透深度的小鼠实时活体脑血管功能成像。此外，IR-820成功用于体内肿瘤的NIR-II荧光成像和光热治疗，皮下肿瘤得到明显抑制或完全根除。结论：由于IR-820在NIR-II光谱区具有相当大的荧光强度和良好的光热效应，生物相容性和可排泄性IR-820在临床实践中的功能性血管造影和癌症治疗诊断方面具有巨大的潜力。

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Excretable IR-820 for in vivo NIR-II fluorescence cerebrovascular imaging and photothermal therapy of subcutaneous tumor.

Rationale: Cerebrovascular diseases, together with malignancies, still pose a huge threat to human health nowadays. With the advantages of its high spatial resolution and large penetration depth, fluorescence bioimaging in the second near-infrared spectral region (NIR-II, 900-1700 nm) and its related imaging-guided therapy based on biocompatible fluorescence dyes have become a promising theranostics method. Methods: The biocompatibility of IR-820 we used in NIR-II fluorescence bioimaging was verified by long-term observation. The model of the mouse with a cranial window, the mouse model of middle cerebral artery occlusion (MCAO) and a subcutaneous xenograft mouse model of bladder tumor were established. NIR-II fluorescence cerebrovascular functional imaging was carried out by IR-820 assisted NIR-II fluorescence microscopy. Bladder tumor was treated by NIR-II fluorescence imaging-guided photothermal therapy. Results: We have found that IR-820 has considerable NIR-II fluorescence intensity, and shows increased brightness in serum than in water. Herein, we achieved real time and in vivo cerebrovascular functional imaging of mice with high spatial resolution and large penetration depth, based on IR-820 assisted NIR-II fluorescence microscopy. In addition, IR-820 was successfully employed for NIR-II fluorescence imaging and photothermal therapy of tumor in vivo, and the subcutaneous tumors were inhibited obviously or eradicated completely. Conclusion: Due to the considerable fluorescence intensity in NIR-II spectral region and the good photothermal effect, biocompatible and excretable IR-820 holds great potentials for functional angiography and cancer theranostics in clinical practice.

更新日期：2019-08-07

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