Surface modification by conjugating biomolecules has been widely proved to enhance biocompatibility of small-caliber artificial vascular grafts. In this study, we aimed at developing a multifunctional vascular graft that provides not only good hemocompatibility but also in situ rapid endothelialization. Herein, a vascular graft (inner diameter ∼2 mm) was fabricated by electrospinning with poly(lactic acid-co-caprolactone) and gelatin, and then biofunctionalized with antithrombotic peptide with sequence LTFPRIVFVLG (ACH₁₁) and cell adhesion peptide with sequence CAG through adhesive poly(dopamine) coating. We developed this graft with the synergistic properties of low thrombogenicity and rapid endothelialization. The successful grafting of both CAG and ACH₁₁ peptides was confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The surface micromorphology of the modified surfaces was observed by field emission scanning electron microscopy. Our results demonstrated that the multifunctional surface suppressed the denaturation of absorbed fibrinogen, hindered coagulation factor Xa activation, and inhibited platelet adhesion and aggregation. Importantly, this modified surface could selectively enhance endothelial cells adhesion, proliferation and release of nitric oxide. Upon in vivo implantation of 6 weeks, the multifunctional vascular graft showed improved patency and superior vascular endothelialization. Overall, the results effectively demonstrated that the co-immobilization of ACH₁₁ and CAG provided a promising method for the improvement of hemocompatibility and endothelialization of vascular grafts.

通过共轭生物分子进行的表面修饰已被广泛证明可增强小口径人工血管移植物的生物相容性。在这项研究中，我们旨在开发一种多功能血管移植物，该移植物不仅提供良好的血液相容性，而且还提供原位快速内皮化。在此，通过用聚乳酸-己内酯和明胶进行电纺丝来制造血管移植物（内径约2 mm），然后通过序列为LTFPRIVFVLG（ACH ₁₁）的抗血栓肽和序列为CAG的细胞粘附肽进行生物功能化。粘性聚（多巴胺）涂料。我们开发了具有低血栓形成性和快速内皮化的协同特性的这种移植物。CAG和ACH ₁₁的成功嫁接通过傅里叶变换红外光谱法和X射线光电子能谱法证实了这些肽。通过场发射扫描电子显微镜观察改性表面的表面微观形态。我们的结果表明，多功能表面抑制了吸收的纤维蛋白原的变性，阻碍了凝血因子Xa的活化，并抑制了血小板的黏附和聚集。重要的是，该修饰的表面可以选择性地增强内皮细胞的粘附，一氧化氮的增殖和释放。在体内植入6周后，多功能血管移植物显示出改善的通畅性和优异的血管内皮化。总体而言，结果有效地证明了ACH ₁₁的共同固定化 CAG为改善血管移植物的血液相容性和内皮化提供了一种有前途的方法。