Pain management using photobiomodulation: Mechanisms, location, and repeatability quantified by pain threshold and neural biomarkers in mice,Journal of Biophotonics

当前位置： X-MOL 学术 › J. Biophotonics › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Pain management using photobiomodulation: Mechanisms, location, and repeatability quantified by pain threshold and neural biomarkers in mice
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2018-05-18 , DOI: 10.1002/jbio.201700370
Marcelo Victor Pires de Sousa _{1,

2,

3} , Masayoshi Kawakubo _{1,

4} , Cleber Ferraresi _{1,

5,

6,

7} , Beatriz Kaippert _{1,

8,

9} , Elisabeth Mateus Yoshimura ₃ , Michael R. Hamblin _{1,

4,

10}

Affiliation

Wellman Center for Photomedicine, Massachusetts General Hospital; Boston Massachusetts
Bright Photomedicine Ltd; São Paulo Brazil
Laboratory of Radiation Dosimetry and Medical Physics, Institute of Physics; University of São Paulo; São Paulo Brazil
Department of Dermatology; Harvard Medical School; Boston Massachusetts
Post-Graduation Program in Biotechnology; Federal University of São Carlos; São Paulo Brazil
Post-Graduation in Physical Therapy in Functional Health, Physical Therapy Department; Universidade do Sagrado Coração; São Paulo Brazil
Post-Graduation Program in Biomedical Engineering; Universidade Brasil; São Paulo Brazil
Pharmacy School; Federal University of Rio de Janeiro; Rio de Janeiro Brazil
Clinical Research Platform; Vice-Presidency of Research and Biological Collections, Oswaldo Cruz Foundation; Rio de Janeiro Brazil
Harvard-MIT Division of Health Sciences and Technology; Cambridge Massachusetts

Photobiomodulation (PBM) is a simple, efficient and cost‐effective treatment for both acute and chronic pain. We previously showed that PBM applied to the mouse head inhibited nociception in the foot. Nevertheless, the optimum parameters, location for irradiation, duration of the effect and the mechanisms of action remain unclear. In the present study, the pain threshold in the right hind paw of mice was studied, after PBM (810 nm CW laser, spot size 1 or 6 cm², 1.2–36 J/cm²) applied to various anatomical locations. The pain threshold, measured with von Frey filaments, was increased more than 3‐fold by PBM to the lower back (dorsal root ganglion, DRG), as well as to other neural structures along the pathway such as the head, neck and ipsilateral (right) paw. On the other hand, application of PBM to the contralateral (left) paw, abdomen and tail had no effect. The optimal effect occurred 2 to 3 hours post‐PBM and disappeared by 24 hours. Seven daily irradiations showed no development of tolerance. Type 1 metabotropic glutamate receptors decreased, and prostatic acid phosphatase and tubulin‐positive varicosities were increased as shown by immunofluorescence of DRG samples. These findings elucidate the mechanisms of PBM for pain and provide insights for clinical practice.

中文翻译：

使用光生物调节的疼痛管理：通过小鼠的疼痛阈值和神经生物标记物量化的机制，位置和可重复性

光生物调节（PBM）是一种针对急性和慢性疼痛的简单，有效且具有成本效益的治疗方法。我们以前表明，将PBM应用于小鼠头部可抑制脚的伤害感受。然而，最佳参数，照射位置，作用持续时间和作用机理仍不清楚。在本研究中，在小鼠的右后爪的疼痛阈值进行了研究，PBM后（810纳米CW激光，光点尺寸1或6厘米²，1.2-36焦耳/厘米²）应用于各种解剖位置。用von Frey细丝测量的疼痛阈值通过PBM向腰下部（背根神经节，DRG）以及沿路径的其他神经结构（如头，颈部和同侧）的作用增加了3倍以上（右）爪子。另一方面，将PBM应用于对侧（左）爪，腹部和尾巴没有影响。最佳效果发生在PBM后2至3小时，到24小时就消失了。每天七次照射未显示出耐受性。DRG样品的免疫荧光显示，1型代谢型谷氨酸受体减少，前列腺酸磷酸酶和微管蛋白阳性静脉曲张增加。这些发现阐明了PBM缓解疼痛的机制，并为临床实践提供了见识。

更新日期：2018-05-18

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南