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Plant cell-surface GIPC sphingolipids sense salt to trigger Ca2+ influx
Nature ( IF 50.5 ) Pub Date : 2019-07-31 , DOI: 10.1038/s41586-019-1449-z Zhonghao Jiang 1, 2, 3, 4 , Xiaoping Zhou 3 , Ming Tao 1 , Fang Yuan 2, 3 , Lulu Liu 2, 3 , Feihua Wu 1, 2, 3 , Xiaomei Wu 3 , Yun Xiang 2 , Yue Niu 2 , Feng Liu 2 , Chijun Li 2 , Rui Ye 2 , Benjamin Byeon 2 , Yan Xue 2 , Hongyan Zhao 3 , Hsin-Neng Wang 4, 5 , Bridget M Crawford 4, 5 , Douglas M Johnson 6 , Chanxing Hu 2 , Christopher Pei 2 , Wenming Zhou 1 , Gary B Swift 6 , Han Zhang 7 , Tuan Vo-Dinh 4, 5 , Zhangli Hu 1 , James N Siedow 2 , Zhen-Ming Pei 2
Nature ( IF 50.5 ) Pub Date : 2019-07-31 , DOI: 10.1038/s41586-019-1449-z Zhonghao Jiang 1, 2, 3, 4 , Xiaoping Zhou 3 , Ming Tao 1 , Fang Yuan 2, 3 , Lulu Liu 2, 3 , Feihua Wu 1, 2, 3 , Xiaomei Wu 3 , Yun Xiang 2 , Yue Niu 2 , Feng Liu 2 , Chijun Li 2 , Rui Ye 2 , Benjamin Byeon 2 , Yan Xue 2 , Hongyan Zhao 3 , Hsin-Neng Wang 4, 5 , Bridget M Crawford 4, 5 , Douglas M Johnson 6 , Chanxing Hu 2 , Christopher Pei 2 , Wenming Zhou 1 , Gary B Swift 6 , Han Zhang 7 , Tuan Vo-Dinh 4, 5 , Zhangli Hu 1 , James N Siedow 2 , Zhen-Ming Pei 2
Affiliation
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Salinity is detrimental to plant growth, crop production and food security worldwide. Excess salt triggers increases in cytosolic Ca2+ concentration, which activate Ca2+-binding proteins and upregulate the Na+/H+ antiporter in order to remove Na+. Salt-induced increases in Ca2+ have long been thought to be involved in the detection of salt stress, but the molecular components of the sensing machinery remain unknown. Here, using Ca2+-imaging-based forward genetic screens, we isolated the Arabidopsis thaliana mutant monocation-induced [Ca2+]iincreases 1 (moca1), and identified MOCA1 as a glucuronosyltransferase for glycosyl inositol phosphorylceramide (GIPC) sphingolipids in the plasma membrane. MOCA1 is required for salt-induced depolarization of the cell-surface potential, Ca2+ spikes and waves, Na+/H+ antiporter activation, and regulation of growth. Na+ binds to GIPCs to gate Ca2+ influx channels. This salt-sensing mechanism might imply that plasma-membrane lipids are involved in adaption to various environmental salt levels, and could be used to improve salt resistance in crops.The sphingolipid GIPC in the plant cell plasma membrane binds to sodium and triggers calcium influx, thereby triggering responses to excess salt such as efflux of sodium ions from cells.
中文翻译：
植物细胞表面 GIPC 鞘脂感知盐以触发 Ca2+ 流入
盐度对全世界的植物生长、作物生产和粮食安全都是有害的。过量的盐会触发细胞溶质 Ca2+ 浓度的增加，从而激活 Ca2+ 结合蛋白并上调 Na+/H+ 逆向转运蛋白以去除 Na+。长期以来，人们一直认为盐诱导的 Ca2+ 增加与盐胁迫的检测有关，但传感机制的分子成分仍然未知。在这里，使用基于 Ca2+ 成像的正向遗传筛选，我们分离了拟南芥突变体单阳离子诱导的 [Ca2+]i 增加 1 (moca1)，并将 MOCA1 鉴定为质膜中糖基肌醇磷酸神经酰胺 (GIPC) 鞘脂的葡萄糖醛酸基转移酶。MOCA1 是盐诱导的细胞表面电位去极化、Ca2+ 尖峰和波、Na+/H+ 逆向转运体激活所必需的，和生长调节。Na+ 与 GIPC 结合以控制 Ca2+ 流入通道。这种盐感应机制可能意味着质膜脂质参与适应各种环境盐水平，并可用于提高作物的耐盐性。植物细胞质膜中的鞘脂 GIPC 与钠结合并引发钙内流，从而触发对过量盐的反应，例如钠离子从细胞中流出。
更新日期：2019-07-31
中文翻译：
植物细胞表面 GIPC 鞘脂感知盐以触发 Ca2+ 流入
盐度对全世界的植物生长、作物生产和粮食安全都是有害的。过量的盐会触发细胞溶质 Ca2+ 浓度的增加，从而激活 Ca2+ 结合蛋白并上调 Na+/H+ 逆向转运蛋白以去除 Na+。长期以来，人们一直认为盐诱导的 Ca2+ 增加与盐胁迫的检测有关，但传感机制的分子成分仍然未知。在这里，使用基于 Ca2+ 成像的正向遗传筛选，我们分离了拟南芥突变体单阳离子诱导的 [Ca2+]i 增加 1 (moca1)，并将 MOCA1 鉴定为质膜中糖基肌醇磷酸神经酰胺 (GIPC) 鞘脂的葡萄糖醛酸基转移酶。MOCA1 是盐诱导的细胞表面电位去极化、Ca2+ 尖峰和波、Na+/H+ 逆向转运体激活所必需的，和生长调节。Na+ 与 GIPC 结合以控制 Ca2+ 流入通道。这种盐感应机制可能意味着质膜脂质参与适应各种环境盐水平，并可用于提高作物的耐盐性。植物细胞质膜中的鞘脂 GIPC 与钠结合并引发钙内流，从而触发对过量盐的反应，例如钠离子从细胞中流出。
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