Ratiometric emission NIR-fluorescent probe for the detection of lysosomal pH in living cells and in vivo†,New Journal of Chemistry

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Ratiometric emission NIR-fluorescent probe for the detection of lysosomal pH in living cells and in vivo†
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2019-07-31 00:00:00 , DOI: 10.1039/c9nj02771h
Weifen Niu _{1,

2,

3,

4} , Juan Jia _{1,

2,

3,

4} , Junkai Li _{1,

2,

3,

4} , Chao Zhang _{1,

2,

3,

4} , Keming Yun _{1,

2,

3,

4}

Affiliation

The lysosome is known to be an acidic organelle that is ubiquitous in cells. Acidic pH is a characteristic feature of lysosomes, and aberrant lysosomal pH values have been manifested to be associated with diverse diseases. In order to monitor the lysosomal pH changes, we developed a ratiometric emission NIR-fluorescent probe (CzQl) for the quantitative analysis of the lysosomal pH in living cells and in vivo. CzQl was constructed via the ethylene bridging of carbazole derivatives and quinoline. The protonation of the probe resulted in a marked green-to-red emission color change. The probe exhibited an ideal pK_a value of 4.60 with a linear relationship in the pH range of 3.9–5.3, which covered most of the pH ranges of lysosomes, and was useful for the quantitative detection and imaging of lysosomal pH changes. Moreover, CzQl exhibited easy loading capability, high selectivity, a large Stokes shift, specific lysosome-targeting ability and low cytotoxicity; thereby, it was successfully used for monitoring the lysosomal pH changes at a cellular level as well as the LPS-mediated inflammation in vivo.

中文翻译：

比例发射近红外荧光探针可检测活细胞和体内的溶酶体pH †

已知溶酶体是在细胞中普遍存在的酸性细胞器。酸性pH是溶酶体的特征性特征，并且溶酶体的pH值异常已被证明与多种疾病有关。为了监测溶酶体的pH变化，我们开发了一种比例发射NIR荧光探针（CzQl），用于定量分析活细胞和体内溶酶体的pH 。CzQ1是通过咔唑衍生物和喹啉的乙烯桥联而构建的。探针的质子化导致显着的绿色到红色发射颜色变化。探针表现出理想的p K _apH值为4.60，在3.9-5.3的pH范围内呈线性关系，涵盖了大部分的溶酶体pH范围，可用于溶酶体pH变化的定量检测和成像。而且，CzQ1表现出容易的负载能力，高选择性，大的斯托克斯位移，特异性的溶酶体靶向能力和低的细胞毒性。因此，它已成功用于在细胞水平上监测溶酶体的pH变化以及体内LPS介导的炎症。

更新日期：2019-07-31

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