酶修饰的异槲皮苷通过激活雄性C57BL / 6小鼠中的AMPKα促进能量代谢。,Food & Function

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酶修饰的异槲皮苷通过激活雄性C57BL / 6小鼠中的AMPKα促进能量代谢。
Food & Function ( IF 5.1 ) Pub Date : 2019-08-05 , DOI: 10.1039/c9fo01008d
Hao Jiang ₁ , Yasukiyo Yoshioka , Sihao Yuan , Yuko Horiuchi , Yoko Yamashita , Kevin D Croft , Hitoshi Ashida

Affiliation

槲皮素具有多种有益健康的功能，但其生物利用度差限制了这些功能。酶修饰的异槲皮苷（EMIQ）是一种槲皮素糖苷，具有比槲皮素更高的生物利用度。在这项研究中，我们调查了EMIQ是否调节小鼠的能量代谢及其潜在的分子机制。给雄性C57BL / 6小鼠喂食正常饮食，并给予不同剂量的EMIQ或槲皮素（0.02％，0.1％和0.5％）两周。补充0.1％EMIQ可显着降低白色脂肪组织（WAT）的重量。补充0.02％和0.1％EMIQ可以促进WAT，肝脏和肌肉中单磷酸腺苷活化蛋白激酶（AMPK）的磷酸化。在WAT中，0.1％EMIQ下调了过氧化物酶体增殖物激活受体（PPAR）γ，CCAAT增强子结合蛋白（C / EBP）α，C /EBPβ，和固醇调节元件结合蛋白1的表达，以及线粒体解偶联蛋白（UCP）2和肉碱棕榈酰转移酶1的表达上调。补充0.1％EMIQ还可以促进WAT中与热生成相关因子的表达，包括PPARγ辅助激活因子α（PGC-1α），UCP1，含PR结构域的蛋白16和sirtuin 1。在肝脏中，EMIQ促进了乙酰辅酶A羧化酶的磷酸化，并增加了PPARα，组成型雄烷受体和法呢素X受体的表达。此外，补充0.02％或0.1％的EMIQ抑制了血浆葡萄糖水平，伴随着葡萄糖转运蛋白4向肌肉质膜的移位。

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Enzymatically modified isoquercitrin promotes energy metabolism through activating AMPKα in male C57BL/6 mice.

Quercetin possesses various health beneficial functions, but its poor bioavailability limits these functions. Enzymatically modified isoquercitrin (EMIQ) is a quercetin glycoside with a greater bioavailability than quercetin. In this study, we investigated whether EMIQ regulates energy metabolism in mice and its underlying molecular mechanism. Male C57BL/6 mice were fed a normal diet with different doses of EMIQ or quercetin (0.02%, 0.1% and 0.5%) for two weeks. Supplementation with 0.1% EMIQ significantly decreased white adipose tissue (WAT) weight. Supplementation with 0.02% and 0.1% EMIQ promoted phosphorylation of adenosine monophosphate activated protein kinase (AMPK) in the WAT, liver, and muscle. In the WAT, 0.1% EMIQ downregulated peroxisome proliferator-activated receptor (PPAR)γ, CCAAT-enhancer-binding protein (C/EBP)α, C/EBPβ, and sterol regulatory element-binding protein 1 expression, as well as upregulated mitochondrial uncoupling protein (UCP) 2 and carnitine palmitoyltransferase-1 expression. Supplementation with 0.1% EMIQ also promoted the expression of thermogenesis-associated factors including PPARγ coactivator α (PGC-1α), UCP1, PR-domain containing protein 16, and sirtuin 1 in the WAT. In the liver, EMIQ promoted the phosphorylation of acetyl-CoA carboxylase, and increased the expression of PPARα, constitutive androstane-receptor, and farnesoid X receptor. Furthermore, supplementation with 0.02% or 0.1% EMIQ suppressed the plasma glucose level accompanied by the translocation of glucose transporter 4 to the plasma membrane of the muscle. Our results suggest that EMIQ is a potential food additive for the regulation of energy metabolism through AMPK phosphorylation.

更新日期：2019-07-31

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