Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung.,Nature Communications

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Genetically diverse Pseudomonas aeruginosa populations display similar transcriptomic profiles in a cystic fibrosis explanted lung.
Nature Communications ( IF 14.7 ) Pub Date : 2019-07-30 , DOI: 10.1038/s41467-019-11414-3
Adrian Kordes ₁ , Matthias Preusse ₂ , Sven D Willger _{1,

2} , Peter Braubach _{3,

4} , Danny Jonigk _{3,

4} , Axel Haverich _{4,

5} , Gregor Warnecke _{4,

5} , Susanne Häussler _{1,

2}

Affiliation

Previous studies have demonstrated substantial genetic diversification of Pseudomonas aeruginosa across sub-compartments in cystic fibrosis (CF) lungs. Here, we isolate P. aeruginosa from five different sampling areas in the upper and lower airways of an explanted CF lung, analyze ex vivo transcriptional profiles by RNA-seq, and use colony re-sequencing and deep population sequencing to determine the genetic diversity within and across the various sub-compartments. We find that, despite genetic variation, the ex vivo transcriptional profiles of P. aeruginosa populations inhabiting different regions of the CF lung are similar. Although we cannot estimate the extent to which the transcriptional response recorded here actually reflects the in vivo transcriptomes, our results indicate that there may be a common in vivo transcriptional profile in the CF lung environment.

中文翻译：

遗传多样性的铜绿假单胞菌群体在囊性纤维化移植肺中表现出相似的转录组特征。

先前的研究已经证明，囊性纤维化（CF）肺的亚区室中铜绿假单胞菌具有显着的遗传多样性。在这里，我们从外植 CF 肺的上呼吸道和下呼吸道的五个不同采样区域中分离出铜绿假单胞菌，通过 RNA-seq 分析离体转录谱，并使用菌落重新测序和深度群体测序来确定内部的遗传多样性。以及各个子隔间。我们发现，尽管存在遗传变异，居住在 CF 肺不同区域的铜绿假单胞菌种群的离体转录谱是相似的。虽然我们无法估计此处记录的转录反应实际上反映体内转录组的程度，但我们的结果表明，CF 肺环境中可能存在共同的体内转录谱。

更新日期：2019-07-30

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