Abstract Enzyme mimicking materials have attracted significant interest due to their ability to replace expensive and unstable enzymes, in a range of applications. The present study is focused on an effective and economic metal salt that mimics enzymatic catalysts, in oxidising D-glucose, for glucose analysis. This enzyme mimicking salt, termed as ‘salzyme’, mimics the activity of D-glucose oxidase, by converting D-glucose to D-gluconic acid, along with spontaneous formation of silver nanoparticles (AgNPs) and hydrogen peroxide. The resulting AgNPs were characterized by TEM, for determining their size and morphology and UV spectroscopy for estimation of D-glucose. The detection of hydrogen peroxide produced during D-glucose oxidation was confirmed by dye tests. The results suggest the potential of this enzyme mimicking salt for further exploration in diagnostic and therapeutic applications.

中文翻译：

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原位银纳米颗粒形成作为非酶葡萄糖传感的工具：用酶模拟盐进行研究

摘要 酶模拟材料因其在一系列应用中替代昂贵且不稳定的酶的能力而引起了极大的兴趣。本研究的重点是一种有效且经济的金属盐，它模拟酶催化剂，用于氧化 D-葡萄糖，用于葡萄糖分析。这种酶模拟盐，称为“盐酶”，通过将 D-葡萄糖转化为 D-葡萄糖酸，以及银纳米颗粒 (AgNP) 和过氧化氢的自发形成来模拟 D-葡萄糖氧化酶的活性。所得的 AgNPs 通过 TEM 进行表征，以确定它们的大小和形态，以及用于估计 D-葡萄糖的紫外光谱。D-葡萄糖氧化过程中产生的过氧化氢的检测通过染料试验得到证实。