The dysbiosis of human gut microbiota is strongly associated with the development of colorectal cancer (CRC). The dysbiotic features of the transition from advanced polyp to early-stage CRC are largely unknown. We performed a 16S rRNA gene sequencing and enterotype-based gut microbiota analysis study. In addition to Bacteroides- and Prevotella-dominated enterotypes, we identified an Escherichia-dominated enterotype. We found that the dysbiotic features of CRC were dissimilar in overall samples and especially Escherichia-dominated enterotype. Besides a higher abundance of Fusobacterium, Enterococcus, and Aeromonas in all CRC faecal microbiota, we found that the most notable characteristic of CRC faecal microbiota was a decreased abundance of potential beneficial butyrate-producing bacteria. Notably, Oscillospira was depleted in the transition from advanced adenoma to stage 0 CRC, whereas Haemophilus was depleted in the transition from stage 0 to early-stage CRC. We further identified 7 different CAGs by analysing bacterial clusters. The abundance of microbiota in cluster 3 significantly increased in the CRC group, whereas that of cluster 5 decreased. The abundance of both cluster 5 and cluster 7 decreased in the Escherichia-dominated enterotype of the CRC group. We present the first enterotype-based faecal microbiota analysis. The gut microbiota of colorectal neoplasms can be influenced by its enterotype.

中文翻译：

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沿传统腺瘤-癌结肠直肠癌途径的肠道菌群的基于肠型的分析。

人类肠道菌群的营养不良与结直肠癌（CRC）的发展密切相关。从晚期息肉过渡到早期CRC的不良生物特征在很大程度上是未知的。我们进行了16S rRNA基因测序和基于肠型的肠道菌群分析研究。除了以拟杆菌属和普雷沃菌属为主导的肠型，我们还确定了以大肠杆菌为主导的肠型。我们发现，CRC的不良生物特征在总体样本中尤其是在以大肠杆菌为主导的肠型中是不同的。除了所有CRC粪便微生物群中富集细菌，肠球菌和气单胞菌的含量较高外，我们发现CRC粪便微生物群的最显着特征是潜在的有益丁酸盐生产细菌的含量降低。尤其，在从晚期腺瘤到0期CRC的过渡中消耗了Oscillospira，而在从0期到早期CRC的过渡中消耗了嗜血杆菌。我们通过分析细菌簇进一步鉴定了7种不同的CAG。在CRC组中，簇3中微生物群的丰度显着增加，而簇5中微生物群的丰度降低。CRC组的以大肠杆菌为主的肠型中，簇5和簇7的丰度均降低。我们提出了第一个基于肠型的粪便微生物群分析。大肠肿瘤的肠道菌群可能受其肠型的影响。在CRC组中，簇3中微生物群的丰度显着增加，而簇5中微生物群的丰度降低。在CRC组以大肠杆菌为主的肠型中，簇5和簇7的丰度都降低了。我们提出了第一个基于肠型的粪便微生物群分析。大肠肿瘤的肠道菌群可能受其肠型的影响。在CRC组中，簇3中微生物群的丰度显着增加，而簇5中微生物群的丰度降低。在CRC组以大肠杆菌为主的肠型中，簇5和簇7的丰度都降低了。我们提出了第一个基于肠型的粪便微生物群分析。大肠肿瘤的肠道菌群可能受其肠型的影响。