<br>银屑病关节炎患者激活 CD8+ T 细胞效应器的频率增加。,Scientific Reports

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银屑病关节炎患者激活 CD8+ T 细胞效应器的频率增加。
Scientific Reports ( IF 3.8 ) Pub Date : 2019-07-26 , DOI: 10.1038/s41598-019-47310-5
Marco Diani ₁ , Fabio Casciano ₂ , Laura Marongiu ₃ , Matteo Longhi ₁ , Andrea Altomare ₁ , Paolo D Pigatto _{1,

4} , Paola Secchiero ₂ , Roberto Gambari ₅ , Giuseppe Banfi _{1,

6} , Angelo A Manfredi _{6,

7} , Gianfranco Altomare _{1,

4} , Francesca Granucci ₃ , Eva Reali _{1,

5}

Affiliation

本研究的目的是确定银屑病关节炎患者循环中差异性存在的 T 细胞亚群，并评估它们在外周血和发炎关节之间再循环的可能性。我们分析了 69 名受试者循环 CD8+ 和 CD4+ T 细胞的表型和细胞因子表达：28 名皮肤银屑病患者、15 名银屑病关节炎患者和 26 名健康受试者。在循环中，比较各组间各亚群的百分比，并计算与血清C反应蛋白浓度的相关性。为了研究 T 细胞向发炎关节的迁移，我们对患者血清和滑液进行了 Transwell 迁移测定。在选定的患者中，我们平行分析了外周血和滑液中的 T 细胞。在循环系统中，我们发现银屑病关节炎患者中表达 CD69 的 CD8+ CCR6+ T 细胞效应细胞和产生 IL-17 的 T 细胞的百分比增加。 CD8+效应/效应记忆T细胞向滑液的迁移增加。最后，我们在滑液中发现了 CXCR3+ CD8+ T 细胞和 CD69+ 细胞的积累。两个区室中的 CD4+ T 细胞与 CD8+ T 细胞有许多相似之处。结果表明记忆 T 细胞效应器在银屑病关节炎的全身和关节表现中发挥作用。

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Increased frequency of activated CD8+ T cell effectors in patients with psoriatic arthritis.

The aim of this study is to identify subsets of T cells differentially represented in the circulation of patients with psoriatic arthritis and to evaluate the possibility that they can recirculate between peripheral blood and the inflamed joints. We analyzed the phenotype and cytokine expression in circulating CD8+ and CD4+ T cells in 69 subjects: 28 with cutaneous psoriasis, 15 patients with psoriatic arthritis, and 26 healthy subjects. In the circulation, the percentage of each subset was compared among the groups and correlation was calculated with the serum concentration of C-reactive protein. To investigate the migration of T cells towards the inflamed joints, we performed a transwell migration assay towards patient serum and synovial fluid. In selected patients we analyzed in parallel T cells from peripheral blood and from synovial fluid. In the circulation, we found increased percentage of CD8+ CCR6+ T cell effectors expressing CD69 and of IL-17-producing T cells in patients with psoriatic arthritis. CD8+ effector/effector memory T cells showed increased migration towards synovial fluid. Finally, in synovial fluid we found accumulation of CXCR3+ CD8+ T cells and CD69+ cells. CD4+ T cells in the two compartments shared many similarities with CD8+ T cells. The results indicate a role for memory T cell effectors in systemic and joint manifestations of psoriatic arthritis.

更新日期：2019-07-26

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