Thiol-Gelatin-Norbornene Bioink for Laser-Based High-Definition Bioprinting.,Advanced Healthcare Materials

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Thiol-Gelatin-Norbornene Bioink for Laser-Based High-Definition Bioprinting.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2019-07-26 , DOI: 10.1002/adhm.201900752
Agnes Dobos _{1,

2} , Jasper Van Hoorick _{3,

4} , Wolfgang Steiger _{1,

2} , Peter Gruber _{1,

2} , Marica Markovic _{1,

2} , Orestis G Andriotis _{2,

5} , Andreas Rohatschek _{2,

5} , Peter Dubruel ₃ , Philipp J Thurner _{2,

5} , Sandra Van Vlierberghe _{3,

4} , Stefan Baudis ₆ , Aleksandr Ovsianikov _{1,

2}

Affiliation

Two‐photon polymerization (2PP) is a lithography‐based 3D printing method allowing the fabrication of 3D structures with sub‐micrometer resolution. This work focuses on the characterization of gelatin–norbornene (Gel–NB) bioinks which enables the embedding of cells via 2PP. The high reactivity of the thiol‐ene system allows 2PP processing of cell‐containing materials at remarkably high scanning speeds (1000 mm s⁻¹) placing this technology in the domain of bioprinting. Atomic force microscopy results demonstrate that the indentation moduli of the produced hydrogel constructs can be adjusted in the 0.2–0.7 kPa range by controlling the 2PP processing parameters. Using this approach gradient 3D constructs are produced and the morphology of the embedded cells is observed in the course of 3 weeks. Furthermore, it is possible to tune the enzymatic degradation of the crosslinked bioink by varying the applied laser power. The 3D printed Gel–NB hydrogel constructs show exceptional biocompatibility, supported cell adhesion, and migration. Furthermore, cells maintain their proliferation capacity demonstrated by Ki‐67 immunostaining. Moreover, the results demonstrate that direct embedding of cells provides uniform distribution and high cell loading independently of the pore size of the scaffold. The investigated photosensitive bioink enables high‐definition bioprinting of well‐defined constructs for long‐term cell culture studies.

中文翻译：

硫醇-明胶-降冰片烯生物墨水，用于基于激光的高清生物打印。

双光子聚合（2PP）是一种基于光刻的3D打印方法，可用于制造亚微米分辨率的3D结构。这项工作着眼于明胶-降冰片烯（Gel-NB）生物墨水的表征，该生物墨水能够通过2PP嵌入细胞。硫醇系统的高反应活性使得2PP处理含细胞材料的扫描速度非常快（1000 mm s ^-1）将该技术应用于生物打印领域。原子力显微镜结果表明，通过控制2PP加工参数，可以在0.2–0.7 kPa的范围内调节生成的水凝胶结构的压痕模量。使用这种方法，可以产生3D梯度构建体，并在3周的过程中观察到嵌入细胞的形态。此外，可以通过改变所施加的激光功率来调节交联生物墨水的酶促降解。3D打印的Gel-NB水凝胶结构具有出色的生物相容性，支持的细胞粘附性和迁移能力。此外，细胞可以通过Ki-67免疫染色证明其增殖能力。而且，结果表明细胞的直接包埋提供了均匀的分布和高的细胞负荷，而与支架的孔径无关。经过研究的光敏生物墨水可以对定义明确的构建体进行高清生物打印，以进行长期的细胞培养研究。

更新日期：2019-07-26

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