Antibody-Oligonucleotide Conjugates as Therapeutic, Imaging, and Detection Agents.,Bioconjugate Chemistry

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Antibody-Oligonucleotide Conjugates as Therapeutic, Imaging, and Detection Agents.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2019-07-24 , DOI: 10.1021/acs.bioconjchem.9b00306
Igor Dovgan ₁ , Oleksandr Koniev ₂ , Sergii Kolodych ₂ , Alain Wagner ₁

Affiliation

Antibody-oligonucleotide conjugates (AOCs) are a novel class of synthetic chimeric biomolecules that has been continually gaining traction in different fields of modern biotechnology. This is mainly due to the unique combination of the properties of their two constituents, exceptional targeting abilities and antibody biodistribution profiles, in addition to an extensive scope of oligonucleotide functional and structural roles. Combining these two classes of biomolecules in one chimeric construct has therefore become an important milestone in the development of numerous biotechnological applications, including imaging (DNA-PAINT), detection (PLA, PEA), and therapeutics (targeted siRNA/antisense delivery). Numerous synthetic approaches have been developed to access AOCs ranging from stochastic chemical bioconjugation to site-specific conjugation with reactive handles, introduced into antibody sequences through protein engineering. This Review gives a general overview of the current status of AOC applications with a specific emphasis on the synthetic methods used for their preparation. The reported synthetic techniques are discussed in terms of their practical aspects and limitations. The importance of the development of novel methods for the facile generation of AOCs possessing a defined constitution is highlighted as a priority in AOC research to ensure the advance of their new applications.

中文翻译：

抗体-寡核苷酸结合为治疗剂，成像剂和检测剂。

抗体-寡核苷酸缀合物（AOC）是一类新的合成嵌合生物分子，在现代生物技术的不同领域中不断受到关注。这主要是由于其两个组成部分的特性，优异的靶向能力和抗体生物分布特性的独特组合，以及广泛范围的寡核苷酸功能和结构作用所致。因此，在一个嵌合结构中结合这两类生物分子已成为许多生物技术应用发展的重要里程碑，包括成像（DNA-PAINT），检测（PLA，PEA）和治疗剂（靶向siRNA /反义递送）。已经开发了许多合成方法来访问AOC，从随机化学生物缀合到具有反应性手柄的位点特异性缀合，都已通过蛋白质工程引入了抗体序列。该评论对AOC申请的当前状态进行了总体概述，特别强调了用于制备它们的合成方法。报告的合成技术从实用性和局限性方面进行了讨论。在AOC研究中，优先考虑开发新颖方法以简便地生产具有确定结构的AOC的重要性，以确保其新应用的发展。该评论对AOC申请的当前状态进行了总体概述，特别强调了用于制备它们的合成方法。报告的合成技术从实用性和局限性方面进行了讨论。在AOC研究中，优先考虑开发新颖方法以简便地生产具有确定结构的AOC的重要性，以确保其新应用的发展。该评论对AOC申请的当前状态进行了总体概述，特别强调了用于制备它们的合成方法。报告的合成技术从实用性和局限性方面进行了讨论。在AOC研究中，优先考虑开发新颖方法以简便地生产具有确定结构的AOC的重要性，以确保其新应用的发展。

更新日期：2019-07-25

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