Hyperspectral optical coherence tomography for in vivo visualization of melanin in the retinal pigment epithelium.,Journal of Biophotonics

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Hyperspectral optical coherence tomography for in vivo visualization of melanin in the retinal pigment epithelium.
Journal of Biophotonics ( IF 2.0 ) Pub Date : 2019-08-13 , DOI: 10.1002/jbio.201900153
Danielle J Harper ₁ , Thomas Konegger ₂ , Marco Augustin ₁ , Kornelia Schützenberger ₁ , Pablo Eugui ₁ , Antonia Lichtenegger ₁ , Conrad W Merkle ₁ , Christoph K Hitzenberger ₁ , Martin Glösmann ₃ , Bernhard Baumann ₁

Affiliation

Previous studies for melanin visualization in the retinal pigment epithelium (RPE) have exploited either its absorption properties (using photoacoustic tomography or photothermal optical coherence tomography [OCT]) or its depolarization properties (using polarization sensitive OCT). However, these methods are only suitable when the melanin concentration is sufficiently high. In this work, we present the concept of hyperspectral OCT for melanin visualization in the RPE when the concentration is low. Based on white light OCT, a hyperspectral stack of 27 wavelengths (440‐700 nm) was created in post‐processing for each depth‐resolved image. Owing to the size and shape of the melanin granules in the RPE, the variations in backscattering coefficient as a function of wavelength could be identified—a result which is to be expected from Mie theory. This effect was successfully identified both in eumelanin‐containing phantoms and in vivo in the low‐concentration Brown Norway rat RPE.

中文翻译：

高光谱光学相干断层扫描用于视网膜色素上皮中黑色素的体内可视化。

先前对视网膜色素上皮 (RPE) 中黑色素可视化的研究利用了其吸收特性（使用光声断层扫描或光热光学相干断层扫描 [OCT]）或其去偏振特性（使用偏振敏感 OCT）。然而，这些方法仅适用于黑色素浓度足够高的情况。在这项工作中，我们提出了高光谱 OCT 的概念，用于在浓度较低时 RPE 中黑色素的可视化。基于白光 OCT，在每个深度分辨图像的后处理中创建了 27 个波长（440-700 nm）的高光谱堆栈。由于 RPE 中黑色素颗粒的大小和形状，可以识别反向散射系数随波长变化的变化——这是米氏理论所预期的结果。这种效应在含有真黑素的模型和低浓度棕色挪威大鼠 RPE 体内均得到成功鉴定。

更新日期：2019-08-13

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