AbstractA new dual-emission ratiometric fluorescent probe was synthesized and successfully used for the determination of Malachite Green (MG) in fish farming water. The ratiometric fluorescent probe was successfully composited by sol-gel method using C3N4 and CdTe quantum dots as fluorescent materials combined with mesoporous molecularly imprinted polymers. MG quenches the red fluorescence of the CdTe QDs (with excitation/emission wavelengths at 350/680 nm) while the blue fluorescence of C3N4 (with excitation/emission wavelengths at 350/458 nm) remains unchanged. The change of fluorescence color and fluorescence intensity ratio can be successfully used for quantification of malachite green. In addition, the mesoporous structure has a large surface and good adsorption capacity for malachite green. The normalized intensity of fluorescence increases linearly in the 50–1000 ng·mL−1 MG concentration range, and the detection limit is 10 ng·mL−1. The imprinting factor is 3.2. The nanoprobe was applied to the determination of MG in fish farming water samples. Recoveries and relative standard deviations were 92.5–97.8% and 2.5–6.2%, respectively. Graphical abstractSchematic representation of synthesis of molecularly imprinted mesoporous silica ratiometric fluorescent probes incorporating C3N4 dots and CdTe quantum dots for determination of malachite green.

中文翻译：

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分子印迹介孔二氧化硅结合 C3N4 点和 CdTe 量子点作为比例荧光探针，用于测定孔雀石绿

摘要 合成了一种新型双发射比例荧光探针，并成功用于鱼类养殖水中孔雀石绿（MG）的测定。以C3N4和CdTe量子点为荧光材料，结合介孔分子印迹聚合物，通过溶胶-凝胶法成功合成了比例荧光探针。MG 淬灭 CdTe QD 的红色荧光（激发/发射波长为 350/680 nm），而 C3N4 的蓝色荧光（激发/发射波长为 350/458 nm）保持不变。荧光颜色和荧光强度比的变化可成功用于孔雀石绿的定量。此外，介孔结构对孔雀石绿具有较大的表面积和良好的吸附能力。归一化的荧光强度在 50-1000 ng·mL-1 MG 浓度范围内线性增加，检测限为 10 ng·mL-1。印记因子是3.2。将纳米探针应用于鱼类养殖水样中MG的测定。回收率和相对标准偏差分别为 92.5-97.8% 和 2.5-6.2%。图形摘要合成分子印迹介孔二氧化硅比例荧光探针的示意图，该探针结合了 C3N4 点和 CdTe 量子点，用于测定孔雀石绿。