当前位置：
X-MOL 学术
›
Bioconjugate Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Clustering of Giant Unilamellar Vesicles Promoted by Covalent and Noncovalent Bonding of Functional Groups at Membrane-Embedded Peptides.
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2019-08-07 , DOI: 10.1021/acs.bioconjchem.9b00394 Nicolai Stuhr-Hansen 1 , Charikleia-Despoina Vagianou 1 , Ola Blixt 1
Bioconjugate Chemistry ( IF 4.0 ) Pub Date : 2019-08-07 , DOI: 10.1021/acs.bioconjchem.9b00394 Nicolai Stuhr-Hansen 1 , Charikleia-Despoina Vagianou 1 , Ola Blixt 1
Affiliation
|
Access to clusters of cell-sized globular objects such as giant unilamellar vesicles (GUVs) is of increasing interest due to their potential applications in prototissue and cell-cell adhesion studies. Aggregations of GUVs by four different approaches were observed via covalent as well as noncovalent bond participations of functional groups at membrane embedded cholesterylpeptides using optical microscopy. Passive air oxidation of GUV-surface thiols into trans-GUV disulfide bonds promoted multivesicle aggregation. Aggregations of GUVs into multiclusters were also achieved by introduction of bispyridyl-ligand substituted peptides into GUV-membranes succeeded by rhodium diacetate mediated vesicle clustering and, furthermore, by coinstalling a biotin moiety streptavidin addition attenuating the clustering effect visualized by formation of compact superaggregated GUV-multiclusters. Contacting between two different GUV-populations, i.e., GUV-heteroconnection, was achieved by trans-GUV phenyl ester-hydrazine ligations producing GUV-heteroclusters. Indirectly, GUV-clustering was achieved by strain-promoted azide-alkyne cycloaddition (SPAAC) reacting bicyclononyne (BCN)-GUVs with azido-GlcNAc succeeded by biotinylated wheat germ agglutinin (WGA)-lectin/streptavidin incubation arousing cross-binding of GUVs.
中文翻译：
在膜嵌入肽上通过官能团的共价键和非共价键促进的巨型单层囊泡的聚集。
由于它们在原组织和细胞间粘附研究中的潜在应用，人们越来越关注进入细胞大小的球形物体簇，例如巨型单层囊泡（GUV）。通过光学显微镜，通过膜嵌入的胆甾醇基肽上官能团的共价和非共价键参与，观察到了四种不同方法引起的GUV聚集。GUV表面硫醇的被动空气氧化成反GUV二硫键促进了多囊泡的聚集。通过将双吡啶基-配体取代的肽引入到GUV膜中，也可以实现GUV聚集到多簇中，而双乙酸铑介导的囊泡成簇，并且此外，通过共同安装生物素部分链霉亲和素，可减弱通过形成紧密的超聚集GUV多簇而显现的聚集效果。两个不同的GUV种群之间的接触，即GUV杂合，是通过反式GUV苯酯-肼连接产生GUV杂簇而实现的。间接地，通过菌株促进的叠氮化物-炔烃环加成反应（SPAAC）与双环壬炔（BCN）-GUVs与叠氮基-GlcNAc反应，通过生物素化小麦胚芽凝集素（WGA）-凝集素/链霉亲和素的孵育引起GUV的交叉结合来实现GUV聚类。
更新日期：2019-07-19
中文翻译：
在膜嵌入肽上通过官能团的共价键和非共价键促进的巨型单层囊泡的聚集。
由于它们在原组织和细胞间粘附研究中的潜在应用，人们越来越关注进入细胞大小的球形物体簇，例如巨型单层囊泡（GUV）。通过光学显微镜，通过膜嵌入的胆甾醇基肽上官能团的共价和非共价键参与，观察到了四种不同方法引起的GUV聚集。GUV表面硫醇的被动空气氧化成反GUV二硫键促进了多囊泡的聚集。通过将双吡啶基-配体取代的肽引入到GUV膜中，也可以实现GUV聚集到多簇中，而双乙酸铑介导的囊泡成簇，并且此外，通过共同安装生物素部分链霉亲和素，可减弱通过形成紧密的超聚集GUV多簇而显现的聚集效果。两个不同的GUV种群之间的接触，即GUV杂合，是通过反式GUV苯酯-肼连接产生GUV杂簇而实现的。间接地，通过菌株促进的叠氮化物-炔烃环加成反应（SPAAC）与双环壬炔（BCN）-GUVs与叠氮基-GlcNAc反应，通过生物素化小麦胚芽凝集素（WGA）-凝集素/链霉亲和素的孵育引起GUV的交叉结合来实现GUV聚类。
京公网安备 11010802027423号