<i>体外</i>成牙本质细胞微流控芯片<i></i>,ACS Biomaterials Science & Engineering

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体外成牙本质细胞微流控芯片
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2019-07-18 00:00:00 , DOI: 10.1021/acsbiomaterials.9b00743
Lin Niu ₁ , Hui Zhang _{1,

2,

3} , Yan Liu _{2,

3} , Yijie Wang _{1,

2,

3} , Ang Li ₁ , Ruirui Liu ₁ , Rui Zou ₁ , Qingzhen Yang _{2,

3}

Affiliation

牙本质小管内部生长的成牙本质细胞过程是成牙本质细胞的关键部分，并且它们在牙本质超敏反应中起重要作用。但是，很难体外模拟成牙本质细胞过程的生长，这阻碍了对牙本质过敏和其他牙齿疾病的研究。据我们所知，尚未开发出能够从细胞体外诱导成牙本质细胞过程生长的技术。在当前的研究中，我们通过软光刻技术制造了微流控芯片。芯片上的微通道可以模拟牙本质小管的微结构，连接到微通道的微腔可以用于成牙本质细胞培养。通过使用该芯片，我们成功地诱导了来自细胞体的成牙本质细胞过程的生长。此外，我们设计了具有不同微通道尺寸（即2、4、6和8μm）的芯片，以研究成牙本质细胞过程的生长与微通道施加的几何约束之间的关系。实验结果表明，2μm通道可以成功诱导成牙本质细胞过程的生长。但是，成牙本质细胞会在4、6和8μm的通道中迁移。这一发现表明2μm是诱导成牙本质细胞过程生长的合适大小体外。该值与体内牙本质小管的大小一致。所制造的微流控芯片可作为强大的工具，用于研究将来的成牙本质细胞过程的生理学和病理学，并开发针对牙齿疾病（例如牙本质过敏）的治疗解决方案。

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Microfluidic Chip for Odontoblasts in Vitro

Odontoblast processes, which grow inside dentin tubules, are critical parts of odontoblasts, and they play an important role in dentin hypersensitivity. However, modeling the growth of odontoblast processes in vitro is difficult, which hinders the study of dentin hypersensitivity and other dental diseases. To our knowledge, no technique has yet been developed to induce the growth of odontoblast processes from a cell body in vitro. In the current study, we fabricated a microfluidic chip via soft lithography. The microchannels on the chip can mimic the microstructures of dentin tubules, and the microchambers that connect to the microchannels can be used for odontoblast culture. We successfully induced the growth of odontoblast processes from cell bodies by using this chip. In addition, we designed chips with different microchannel sizes (i.e., 2, 4, 6, and 8 μm) to investigate the relationship between the growth of odontoblast processes and the geometric constraint imposed by microchannels. Experimental results show that the growth of odontoblast processes can be successfully induced by 2 μm channels. However, odontoblasts will migrate in the 4, 6, and 8 μm channels. This finding indicates that 2 μm is the appropriate size for inducing the growth of odontoblast processes in vitro. This value is consistent with the size of dentin tubules in vivo. The fabricated microfluidic chip can serve as a powerful tool for investigating the physiology and pathology of odontoblast processes in the future and developing treatment solutions for dental diseases, such as dentin hypersensitivity.

更新日期：2019-07-18

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