Sesquiterpene lactones (SL) are commonly found in Asteraceae and present a promising anti-inflammatory activity. Previously described in Lepidaploa genus, glaucolide B has never been investigated for its anti-inflammatory potential. This study aimed to establish an efficient process for the extraction of glaucolide B (1) from Lepidaploa chamissonis leaves and to develop a simple and fast method for its purification by using centrifugal partition chromatography (CPC), as well as to investigate in vitro the anti-inflammatory effects of glaucolide B. Thus, an optimized washing extractive process performed on L. chamissonis leaves allowed to obtain a SL enriched extract (4.11 g). After a successful defatting pretreatment of the crude extract, the glaucolide B enriched ethyl acetate portion (2.00 g) was fractionated by CPC affording, in a single-step isolation, compound 1 (1.04 g) in great yield (25%) and purity (97%). Cytotoxicity effect of 1 on RAW 264.7 macrophages was determined by using MTT assay, revealing a CC10 of 14.11 μM. Compound 1 at 1, 3 and 10 μM inhibited the nitrite/nitrate (NOx) metabolites production and the pro-inflammatory interleukin 6 (IL-6) secretion on lipopolysaccharide-stimulated RAW 264.7 cells. The extractive process used turned to be selective for SL and CPC technique proved a simple and effective tool for the isolation of 1 within few hours. Isolated for the first time from L. chamissonis leaves, glaucolide B presented a significant inhibitory effect on both NO and IL-6 secretion under non-toxic concentrations.

中文翻译：

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通过离心分配色谱法一步一步分离潜在的消脂棉沙门氏菌中的消炎性青霉内酯B。

倍半萜内酯（SL）常存在于菊科中，并具有良好的抗炎活性。以前曾在Lepidaploa属中描述过，从未研究过青霉素B的抗炎潜力。这项研究旨在建立一种有效的方法，以从chaponis chapsonis叶片中提取青霉素B（1），并开发一种使用离心分配色谱法（CPC）进行纯化的简单快速的方法，并在体外研究其抗药性。 -青霉素B的炎症作用。因此，对Chamissonis叶进行了优化的洗涤提取过程，从而获得了富含SL的提取物（4.11 g）。在对粗制提取物进行成功的脱脂预处理之后，将富含青草酸B的乙酸乙酯部分（2.00 g）通过CPC进行分馏，得到的 通过一步分离，化合物1（1.04 g）的收率（25％）和纯度（97％）高。使用MTT分析确定1对RAW 264.7巨噬细胞的细胞毒性作用，显示CC10为14.11μM。1、3和10μM的化合物1抑制了脂多糖刺激的RAW 264.7细胞上亚硝酸盐/硝酸盐（NOx）代谢产物的产生和促炎性白介素6（IL-6）的分泌。提取工艺对SL和CPC技术具有选择性，证明了在数小时内分离出1的简单有效的工具。首次从chamissonis叶片中分离出的青草酸酯B在无毒浓度下对NO和IL-6分泌均表现出显着的抑制作用。通过使用MTT分析确定了7个巨噬细胞，显示出10.11μM的CC10。1、3和10μM的化合物1抑制了脂多糖刺激的RAW 264.7细胞上亚硝酸盐/硝酸盐（NOx）代谢产物的产生和促炎性白介素6（IL-6）的分泌。提取工艺对SL和CPC技术具有选择性，证明了在数小时内分离出1的简单有效的工具。首次从chamissonis叶片中分离出的青草酸酯B在无毒浓度下对NO和IL-6分泌均表现出显着的抑制作用。通过使用MTT分析确定了7个巨噬细胞，显示出10.11μM的CC10。1、3和10μM的化合物1抑制了脂多糖刺激的RAW 264.7细胞上亚硝酸盐/硝酸盐（NOx）代谢产物的产生和促炎性白介素6（IL-6）的分泌。提取工艺对SL和CPC技术具有选择性，证明了在数小时内分离出1的简单有效的工具。首次从chamissonis叶片中分离出的青草酸酯B在无毒浓度下对NO和IL-6分泌均表现出显着的抑制作用。提取工艺对SL和CPC技术具有选择性，证明了在数小时内分离出1的简单有效的工具。首次从chamissonis叶片中分离出的青草酸酯B在无毒浓度下对NO和IL-6分泌均表现出显着的抑制作用。提取工艺对SL和CPC技术具有选择性，证明了在数小时内分离出1的简单有效的工具。首次从chamissonis叶片中分离出的青草酸酯B在无毒浓度下对NO和IL-6分泌均表现出显着的抑制作用。