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Selective Autophagy: ATG8 Family Proteins, LIR Motifs and Cargo Receptors.
Journal of Molecular Biology ( IF 4.7 ) Pub Date : 2019-07-13 , DOI: 10.1016/j.jmb.2019.07.016
Terje Johansen 1 , Trond Lamark 1
Affiliation  

Selective autophagy relies on soluble or membrane-bound cargo receptors that recognize cargo and bring about autophagosome formation at the cargo. The cargo-bound receptors interact with lipidated ATG8 family proteins anchored in the membrane at the concave side of the forming autophagosome. The interaction is mediated by 15- to 20-amino-acid-long sequence motifs called LC3-interacting region (LIR) motifs that bind to the LIR docking site (LDS) of ATG8 proteins. In this review, we focus on LIR-ATG8 interactions and the soluble mammalian selective autophagy receptors. We discuss the roles of ATG8 family proteins as membrane scaffolds in autophagy and the LIR-LDS interaction and how specificity for binding to GABARAP or LC3 subfamily proteins is achieved. We also discuss atypical LIR-LDS interactions and a novel LIR-independent interaction. Recently, it has become clear that several of the soluble cargo receptors are able to recruit components of the core autophagy apparatus to aid in assembling autophagosome formation at the site of cargo sequestration. A model on phagophore recruitment and expansion on a selective autophagy receptor-coated cargo incorporating the latest findings is presented.

中文翻译:

选择性自噬:ATG8家族蛋白,LIR基序和货物受体。

选择性自噬依赖于识别货物并在货物上引起自噬体形成的可溶性或膜结合货物受体。货物结合的受体与锚定在形成自噬体凹面膜中的脂化ATG8家族蛋白相互作用。相互作用是由15至20个氨基酸长的序列基序(称为LC3相互作用区(LIR)基序)介导的,该基序与ATG8蛋白的LIR停靠位点(LDS)结合。在这篇综述中,我们专注于LIR-ATG8相互作用和可溶性哺乳动物选择性自噬受体。我们讨论了ATG8家族蛋白在自噬和LIR-LDS相互作用中作为膜支架的作用,以及如何实现与GABARAP或LC3亚家族蛋白结合的特异性。我们还将讨论非典型LIR-LDS相互作用和一种新型的LIR独立相互作用。最近,已经清楚的是,几种可溶性货物受体能够募集核心自噬装置的成分,以帮助在货物螯合位点组装自噬体的形成。提出了结合最新发现的选择性自噬受体涂层货物的荧光团募集和扩展模型。
更新日期:2020-01-15
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