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Comparison of Cytotoxicity Evaluation of Anticancer Drugs between Real-Time Cell Analysis and CCK-8 Method
ACS Omega ( IF 3.7 ) Pub Date : 2019-07-11 00:00:00 , DOI: 10.1021/acsomega.9b01142 Ling Cai 1 , Xijiang Qin 1 , Zhihui Xu 1 , Yiyan Song 1 , Huijun Jiang 1 , Yuan Wu 2 , Hongjie Ruan 3 , Jin Chen 1, 4, 4
ACS Omega ( IF 3.7 ) Pub Date : 2019-07-11 00:00:00 , DOI: 10.1021/acsomega.9b01142 Ling Cai 1 , Xijiang Qin 1 , Zhihui Xu 1 , Yiyan Song 1 , Huijun Jiang 1 , Yuan Wu 2 , Hongjie Ruan 3 , Jin Chen 1, 4, 4
Affiliation
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Critical cytotoxicity evaluation of pharmaceuticals is necessary for the clinical practice of chemotherapy. To quantitatively evaluate cell viability, currently there are two main types of sensitive methods including real-time cell analysis (RTCA) and CCK-8 assay, in which RTCA records electrochemical signal changes around an incubated cell, whereas CCK-8 is based on the colorimetric method. Despite the different detection principles adopted for the cytotoxicity assessment, the comparison of the two methods in terms of the application scope is lacking. In this study, comparison studies were conducted between the RTCA and CCK-8 assays using anticancer drugs including doxorubicin hydrochloride, curcumin, irinotecan (CPT-11), taxol, and oxaliplatin, which are classified into two groups of drug molecules in the absence and presence of additives. The cytotoxicity evaluation of these drugs on cancer cells revealed that the physicochemical properties of drug formulations such as optical and electrochemical properties are closely linked with the readout of cytotoxic methods. The experimental results suggested that the preselection of cytotoxic assay is critical for the quantitative measurement of cytotoxicity of anticancer drugs, which is of clinical importance for their therapeutic usage.
中文翻译：
实时细胞分析与CCK-8法抗癌药物细胞毒性评价的比较
药物的关键细胞毒性评价对于化疗的临床实践是必要的。为了定量评估细胞活力，目前主要有两种敏感方法，包括实时细胞分析（RTCA）和CCK-8测定，其中RTCA记录孵育细胞周围的电化学信号变化，而CCK-8是基于比色法。尽管细胞毒性评估采用的检测原理不同，但缺乏两种方法在应用范围方面的比较。在本研究中，使用盐酸阿霉素、姜黄素、伊立替康 (CPT-11)、紫杉醇和奥沙利铂等抗癌药物进行 RTCA 和 CCK-8 测定法之间的比较研究，这些药物分子分为两组药物分子，有无和有无。添加剂的存在。这些药物对癌细胞的细胞毒性评估表明，药物制剂的理化性质（例如光学和电化学性质）与细胞毒性方法的读数密切相关。实验结果表明，细胞毒性测定的预选对于抗癌药物细胞毒性的定量测量至关重要，这对其治疗用途具有临床意义。
更新日期：2019-07-11
中文翻译：
实时细胞分析与CCK-8法抗癌药物细胞毒性评价的比较
药物的关键细胞毒性评价对于化疗的临床实践是必要的。为了定量评估细胞活力，目前主要有两种敏感方法，包括实时细胞分析（RTCA）和CCK-8测定，其中RTCA记录孵育细胞周围的电化学信号变化，而CCK-8是基于比色法。尽管细胞毒性评估采用的检测原理不同，但缺乏两种方法在应用范围方面的比较。在本研究中，使用盐酸阿霉素、姜黄素、伊立替康 (CPT-11)、紫杉醇和奥沙利铂等抗癌药物进行 RTCA 和 CCK-8 测定法之间的比较研究，这些药物分子分为两组药物分子，有无和有无。添加剂的存在。这些药物对癌细胞的细胞毒性评估表明，药物制剂的理化性质（例如光学和电化学性质）与细胞毒性方法的读数密切相关。实验结果表明，细胞毒性测定的预选对于抗癌药物细胞毒性的定量测量至关重要，这对其治疗用途具有临床意义。
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