协同增强诱导骨分化的聚醚醚酮的生物相容性和生物活性的简便表面改性方法。,ACS Applied Materials & Interfaces

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协同增强诱导骨分化的聚醚醚酮的生物相容性和生物活性的简便表面改性方法。
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2019-07-23 , DOI: 10.1021/acsami.9b03030
Yuchen Zhu ₁ , Zhe Cao , Ying Peng , Liqiu Hu ₂ , Tankut Guney ₁ , Bin Tang

Affiliation

聚醚醚酮（PEEK）由于其合适的机械性能以及出色的耐化学性和生物相容性，在生物医学工程中是一种很有前途的材料。但是，PEEK的生物惰性限制了其应用。在这项研究中，我们开发了一种轻松的浸入方法，以产生可诱导骨分化的生物相容性和生物活性PEEK。首先，先通过浓硫酸引入PEEK表面的微孔，然后将其浸入水中，然后进行水热处理以减少残留的硫酸。随后，通过氧等离子体处理将磺化的PEEK表面活化，然后通过浸入多巴胺溶液中而涂覆聚（多巴胺）（PDA）层。最后，通过浸入RGD肽溶液，将三肽Arg-Gly-Asp（RGD）整合到PEEK涂有PDA的表面上。通过扫描电子显微镜，X射线光电子能谱，水接触角分析，阿基米德流体饱和法，椭圆光度法，石英晶体微天平等系统研究了表面特性（物理化学和生物学特性）以及形成骨状磷灰石的能力。耗散监测，细胞增殖，实时逆转录聚合酶链反应分析，茜素红染色，免疫细胞化学染色和模拟体液浸没。改性PEEK总体上显示出显着提高的促进细胞增殖，成骨分化的能力，与PEEK对照相比，其在体外的骨样磷灰石形成和磷灰石的形成。这些结果表明，PEEK的组合表面改性可增强其生物活性和生物相容性，并诱导骨分化。这项研究提出了一种在骨科植入物的应用中扩大PEEK用途的策略，并且将来可能在工业上推广。

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Facile Surface Modification Method for Synergistically Enhancing the Biocompatibility and Bioactivity of Poly(ether ether ketone) That Induced Osteodifferentiation.

Poly(ether ether ketone) (PEEK) is a promising material in biomedical engineering due to its suitable mechanical properties and excellent chemical resistance and biocompatibility. However, the biological inertness of PEEK limits its applications. In this study, we developed a facile approach of immersion to generate a biocompatible and bioactive PEEK that induced osteodifferentiation. First, micropores on the surface of PEEK were introduced by concentrated sulfuric acid and subsequent water immersion, followed by the hydrothermal treatment to reduce residual sulfuric acid. Subsequently, the sulfonated PEEK surface was activated by the oxygen plasma treatment and then coated with a poly(dopamine) (PDA) layer by immersion into the dopamine solution. Finally, the tripeptide Arg-Gly-Asp (RGD) was integrated onto the PDA-coated surface of PEEK by immersion into the RGD peptide solution. The surface characteristics (physical chemistry and biological properties) and the ability to form bonelike apatite were systematically investigated by scanning electron microscopy, X-ray photoelectron spectroscopy, water contact angle analysis, the Archimedes' fluid saturation method, ellipsometry, a quartz crystal microbalance with dissipation monitoring, cell proliferation, real-time reverse transcription polymerase chain reaction analysis, alizarin red staining, immunocytochemistry staining, and simulated body fluid immersion. Collectively, the modified PEEK showed a significantly improved ability to promote cell proliferation, osteogenic differentiation, and bonelike apatite formation in vitro as compared to the PEEK control. These results demonstrate that combined facile surface modifications for PEEK enhance its bioactivity and biocompatibility, and induce osteodifferentiation. This study presents a strategy for broadening the use of PEEK in the application of orthopedic implants and could be industrially scalable in future.

更新日期：2019-07-10

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