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Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry
ACS Omega ( IF 3.7 ) Pub Date : 2019-07-08 00:00:00 , DOI: 10.1021/acsomega.9b01727 Jorick J Bruins 1 , Criss van de Wouw 1 , Koen Wagner 2 , Lina Bartels 2 , Bauke Albada 1 , Floris L van Delft 1
ACS Omega ( IF 3.7 ) Pub Date : 2019-07-08 00:00:00 , DOI: 10.1021/acsomega.9b01727 Jorick J Bruins 1 , Criss van de Wouw 1 , Koen Wagner 2 , Lina Bartels 2 , Bauke Albada 1 , Floris L van Delft 1
Affiliation
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Knob-in-hole antibodies can be utilized to introduce a single tag for chemo-enzymatic functionalization. By either introducing a single C-terminal sortase tag (sortase-tag expressed protein ligation) or tyrosine tag (G4Y), mono-functionalization of the monoclonal antibody trastuzumab was achieved rapidly and in high yields. This method was applied to selectively and efficiently introduce a single fluorescent tag, cytokine or single-chain variable fragment, as well as produce clean homo dimers of trastuzumab.
中文翻译：
利用应变促进点击化学对旋钮孔抗体进行高效单功能化
旋钮孔抗体可用于引入单个标签以实现化学酶功能化。通过引入单个 C 端分选酶标签（分选酶标签表达的蛋白质连接）或酪氨酸标签 (G 4 Y)，单克隆抗体曲妥珠单抗的单功能化得以快速且高产率地实现。该方法用于选择性、高效地引入单一荧光标签、细胞因子或单链可变片段，以及产生干净的曲妥珠单抗同型二聚体。
更新日期：2019-07-08
中文翻译：
利用应变促进点击化学对旋钮孔抗体进行高效单功能化
旋钮孔抗体可用于引入单个标签以实现化学酶功能化。通过引入单个 C 端分选酶标签（分选酶标签表达的蛋白质连接）或酪氨酸标签 (G 4 Y)，单克隆抗体曲妥珠单抗的单功能化得以快速且高产率地实现。该方法用于选择性、高效地引入单一荧光标签、细胞因子或单链可变片段，以及产生干净的曲妥珠单抗同型二聚体。
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