通过使用经适体和互补 DNA 修饰的磁珠作为捕获探针，以及金属有机框架 MIL-101(Fe) 上的 G-四链体/血红素和 DNA 抗体作为过氧化物酶模拟物，可视化和比色测定猪肉中的瘦肉精,Microchimica Acta

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通过使用经适体和互补 DNA 修饰的磁珠作为捕获探针，以及金属有机框架 MIL-101(Fe) 上的 G-四链体/血红素和 DNA 抗体作为过氧化物酶模拟物，可视化和比色测定猪肉中的瘦肉精
Microchimica Acta ( IF 5.3 ) Pub Date : 2019-07-06 , DOI: 10.1007/s00604-019-3604-5
Yuandong Zhang , Hong-Xia Ren , Yang-Bao Miao

摘要描述了一种用于检测瘦肉精 (CLB) 的可视化策略。它使用标记在 MIL-101(Fe) 型金属有机框架上的抗 dsDNA 和 G-四链体/血红素抗体（G-四链体/血红素-抗-DNA/MIL-101）作为过氧化物酶模拟物，和磁性用适体和互补 DNA (MB/Apt-cDNA) 作为捕获探针修饰的珠子。通过捕获探针中的双链 DNA (Apt-cDNA) 与过氧化物酶模拟物中的抗 DNA 之间的反应制备检测试剂。在 CLB 存在下，磁珠上的适体优先结合 CLB，过氧化物酶模拟物在磁分离后释放到上清液中。释放的过氧化物酶模拟物可以在温和条件下催化 TMB/H2O2 显色系统。这导致出现蓝绿色，其吸光度在 650 nm 处测量。检测限低至 CLB 的 34 fM。该方法用于测定猪肉样品中的 CLB，其结果与使用 ELISA 试剂盒获得的数据一致。图形摘要描述了用于检测瘦肉精的可视化策略。与其他干扰物相比，克仑特罗检测系统的选择性非常好。该方法适用于猪肉样品中CLB的测定。与其他干扰物相比，克仑特罗检测系统的选择性非常好。该方法适用于猪肉样品中CLB的测定。与其他干扰物相比，克仑特罗检测系统的选择性非常好。该方法适用于猪肉样品中CLB的测定。

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Visualization and colorimetric determination of clenbuterol in pork by using magnetic beads modified with aptamer and complementary DNA as capture probes, and G-quadruplex/hemin and DNA antibody on the metal-organic framework MIL-101(Fe) acting as a peroxidase mimic

AbstractA visualization strategy is described for the detection of clenbuterol (CLB). It is using of antibody against dsDNA and G-quadruplex/hemin labeled on a metal organic framework of type MIL-101(Fe) (G-quadruplex/hemin-anti-DNA/MIL-101) acting as a peroxidase mimetic, and magnetic beads modified with aptamer and complementary DNA (MB/Apt-cDNA) as capture probes. The detection reagent was prepared via the reactions between the double stranded DNA (Apt-cDNA) in capture probes and anti-DNA in peroxidase mimetic. In the presence of CLB, the aptamer on the magnetic beads preferentially binds CLB, and the peroxidase mimetic is released to the supernatant after magnetic separation. The released peroxidase mimetic can catalyze the TMB/H2O2 chromogenic system under mild conditions. This leads to the development of a blue-green coloration whose absorbance is measured at 650 nm. The detection limit is as low as 34 fM of CLB. The method was applied to the determination of CLB in pork samples and gave results that were consistent with data obtained with an ELISA kit. Graphical abstractA visualization strategy is described for the detection of clenbuterol. The selectivity of detection system for clenbuterol is excellent compared with other interferents. The method was applied to the determination of CLB in pork samples.

更新日期：2019-07-06

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