Sensitive Naked‐eye and Fluorescence Determination of Acetylcholinesterase Activity using Cu/Ag Nanoclusters Based on Inner Filter Effect,ChemistrySelect

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Sensitive Naked‐eye and Fluorescence Determination of Acetylcholinesterase Activity using Cu/Ag Nanoclusters Based on Inner Filter Effect
ChemistrySelect ( IF 1.9 ) Pub Date : 2019-07-04 , DOI: 10.1002/slct.201901714
Yanyue Li ₁ , Yanling Hu ₁ , Yu He ₁ , Yili Ge ₁ , Gongwu Song ₁ , Jiangang Zhou ₂

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In this work, a novel naked eye and fluorescence sensor for acetylcholinesterase (AChE) determination based on copper/silver nanoclusters (Cu/Ag NCs) was developed. L‐Histidine protected Cu/Ag NCs were prepared and exhibited superior properties compared with copper nanoclusters, with the quantum yield increased from 4.3% to 13.2%. The fluorescence of Cu/Ag NCs can be easily quenched by 2‐nitro‐5‐thiobenzoate anion (TNB) owing to the inner filter effect. TNB is the product between 5′‐Dithiobis‐(2‐nitrobenzoic acid) (DTNB) and thiols. However, AChE can specially catalyze acetylthiocholine (ATCh) to hydrolyze and release biothiols (TCh). Based on this, the determination of AChE was achieved by introducing DTNB. A naked eye sensor was also constructed owing to the color change.

中文翻译：

基于内滤作用的铜/银纳米团簇敏感地通过肉眼和荧光测定乙酰胆碱酯酶活性

在这项工作中，基于铜/银纳米簇（Cu / Ag NCs）的新型肉眼和荧光传感器用于乙酰胆碱酯酶（AChE）的测定。制备了由L-组氨酸保护的Cu / Ag NCs，与铜纳米团簇相比具有更好的性能，量子产率从4.3％增加到13.2％。由于具有内部过滤作用，Cu / Ag NCs的荧光很容易被2-硝基-5-硫代苯甲酸酯阴离子（TNB）猝灭。TNB是5'-二硫代双（2-硝基苯甲酸）（DTNB）和硫醇之间的产物。但是，AChE可以专门催化乙酰硫胆碱（ATCh）水解并释放生物硫醇（TCh）。在此基础上，通过引入DTNB实现了AChE的测定。由于颜色变化，还构造了裸眼传感器。

更新日期：2019-07-04

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