Lactose and Bovine Milk Oligosaccharides Synergistically Stimulate B. longum subsp. longum Growth in a Simplified Model of the Infant Gut Microbiome.,Journal of Proteome Research

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Lactose and Bovine Milk Oligosaccharides Synergistically Stimulate B. longum subsp. longum Growth in a Simplified Model of the Infant Gut Microbiome.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-07-10 , DOI: 10.1021/acs.jproteome.9b00211
Louise M A Jakobsen ₁ , Ulrik K Sundekilde ₁ , Henrik J Andersen ₂ , Dennis S Nielsen ₃ , Hanne C Bertram ₁

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Increasing awareness of the importance of a healthy Bifidobacterium-rich microbiome has led to a need for more knowledge on how different prebiotic carbohydrates specifically impact the infant microbiome, especially as a community instead of single bacterial targets. In this study, we combined proton nuclear magnetic resonance (¹H NMR) metabolomics and molecular biology methods for quantification of bacteria to compare the prebiotic effect of bovine milk oligosaccharides (BMO) and synthetic galacto oligosaccharides (GOS) using mono- and cocultures of eight major bacteria related to a healthy infant microbiome. The results revealed that BMO treatments supported growth of Bifidobacterium longum subsp. longum and Parabacteroides distasonis, while at the same time growth of Clostridium perfringens and Escherichia coli was inhibited. In addition, there was a synergistic effect of combining lactose and BMO in regards to reducing C. perfringens, maintaining stable numbers of P. distasonis and simultaneously increasing numbers of the beneficial B. longum subsp. longum. These results indicate that the oligosaccharide composition plays a vital role in shaping the developing microbiota.

中文翻译：

乳糖和牛乳寡糖协同刺激长双歧杆菌亚种。婴儿肠道微生物组简化模型中的长粒生长。

人们对健康的富含双歧杆菌的微生物组的重要性的认识日益提高，因此需要更多有关不同益生元碳水化合物如何特异性地影响婴儿微生物组的知识，尤其是作为一个社区而不是单个细菌靶标。在这项研究中，我们结合了质子核磁共振（¹ H NMR）代谢组学和分子生物学方法对细菌进行定量，比较了使用8种单培养和共培养的牛低聚糖（BMO）和合成低聚半乳糖（GOS）的益生元效应。与健康的婴儿微生物组有关的主要细菌。结果表明，BMO处理支持长双歧杆菌亚种的生长。长和副细菌副杆菌，同时产气荚膜梭状芽胞杆菌和大肠杆菌的生长受到抑制。另外，结合乳糖和BMO在减少产气荚膜梭菌，保持稳定的数位假单胞菌和同时增加有益的长双歧杆菌亚种的数量方面具有协同作用。长子。这些结果表明，寡糖组合物在形成发育中的微生物群中起着至关重要的作用。

更新日期：2019-07-10

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